TRAINING OBJECTIVES
The Teaching Course of Biochemistry Methodologies aims to provide a theoretical and practical knowledge on the preparative and analytical methods that are mostly used in biochemical research, but also in biomedical and environmental fields.
In particular, the provided knowledge relates to methodologies for the identification, isolation and structural/functional characterization of biological macromolecules, as well as to bioinformatics tools for the analysis of results and for their visualization. Moreover, techniques for analyzing both individual proteins and whole proteomes will be treated.

LEARNING EXPECTED OUTCOMES
1. Knowledge and understanding
At the end of the course the students will be able to:
- know the main techniques used in biochemical investigations (centrifugation, spectroscopy, electrophoresis, chromatography, spectrometry, protein) and the key parameters needed to set up a specific biochemical investigation;
- describe the structural elements of the main instruments of a biochemical laboratory (centrifuge, spectrophotometer, spectrometer);
- to know the appropriate terminology used in biochemical methods.

2. Applying knowledge and understanding
At the end of the course the students will be able to:
- choose the most appropriate biochemical procedure for achieving the experimental goals defined during the design of a research project;
- evaluate the possible impact of variations in the key parameters of a biochemical experiment;
- practically carry out the experiments performed during this course.

3. Making judgements
Students should be able to understand and critically discuss the experimental results obtained in a research laboratory and use them for planning subsequent experiments.

4. Communication skills
Students should have the ability to convey the acquired knowledge in a clear and comprehensible manner, even to people who are not competent, and must demonstrate the ability to present information also with graphs and formulas.

5. Learning skills
Successful condition in learning is the ability to read and understand a scientific paper on biochemistry topic.