APPLIED BIOPHYSICS AND NANOSCIENCES
(objectives)
The teaching of APPLIED BIOPHYSICS AND NANOSCIENCES aims to provide students with basic knowledge in the field of molecular biophysics and some advanced spectroscopic and nanotechnological techniques for applications in the biosensory field. 1) Knowledge and understanding Knowledge of the fundamental principles of molecular biophysics mainly aimed at molecular bio-recognition processes. Knowledge of the theoretical and experimental bases of some advanced spectroscopic and nanotechnological techniques for the study of the structural, dynamic and interaction properties of biological systems. Knowledge of the techniques of quantitative analysis of experimental data in relation to the experimental techniques introduced and of bioinformatics tools with particular attention to bio-recognition processes. 2) Applying knowledge and understanding At the end of the course, students must have acquired a series of knowledge and skills that they will be able to put into practice during the experimental exercises. Students will also be invited to analyze the experimental data obtained using the procedures and methods of analysis presented in the theoretical part. 3) Making judgments During the course, students will be encouraged to critically discuss the topics presented, also looking for links with previous knowledge. In addition, students will be invited to propose and plan possible developments of the experimental activity carried out and the related data analysis. 4) Communication skills During the lessons, students will be invited to express any doubts and to present their vision of the topics under discussion so as to develop their communication skills. In addition, during the experimental activity, students will be invited to work in groups so as to develop the ability to interact with others and work in a team. Finally, through a seminar that will be part of the exam, the ability to present a scientific article in a synthetic, rigorous and effective way will be verified. 5) Learning skills Students will be encouraged to acquire the ability to carry out the various steps that make up the experimental exercises in a conscious and critical way. In addition, they must be able to present the central topics of the course, also relating the various topics to each other. This ability will also be stimulated through the active involvement of students through oral discussions in the classroom and during the hours dedicated to practical exercises.
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Code
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119654 |
Language
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ITA |
Type of certificate
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Profit certificate
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Credits
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6
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Scientific Disciplinary Sector Code
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FIS/07
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Contact Hours
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24
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Laboratory Hours
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24
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Type of Activity
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Related or supplementary learning activities
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Teacher
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BIZZARRI Anna Rita
(syllabus)
The program of the Applied Biophysics and Nanosciences course is centred on the students' acquisition of knowledge and skills in order to better address the vast current problems of biology. Different experimental techniques, some of them innovative, will be presented jointly with applications to various biological systems (proteins, DNA, RNA, and cells) taken from the recent literature. Furthermore, as a central part of the course, students will carry out experimental exercises in the laboratory; the data obtained will then be analyzed and discussed. Below are the techniques that will be presented and related exercises. • Theory and applications of optical absorption spectroscopy. Practical experiments in lab. • Theory and applications of static and time resolved fluorescence, Foester Resonance Energy Transfer (FRET). Practical experiments in lab. • Theory and applications of Raman scattering and Surface Enhanced Raman Spectroscopy (SERS). • Theory and applications of Surface Plasmon Resonance (SPR) for the study of the interaction kinetics between biomolecules. Practical experiments in lab. • Nanoscopy techniques, (Microscopy and Atomic Force Spectroscopy (AFM and AFS)). Practical experiments of imaging in lab by AFM. • Elements of modelling of biological processes. Practical exercises on the computer. • Elements of bio-sensors and nano-sensors for the detection of biomolecules (proteins, DNA and RNA) at low concentrations. Practical experiments in lab.
(reference books)
The slides of the course and - D.A. Skoog, F.J. Holler, T.A. Nieman: “Principles of instrumental analysis”. Harcourt Brace & Company - J.P Allen “Biophysical Chemistry” Wiley-Blackwell
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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Mandatory
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Evaluation methods
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Oral exam
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