Teacher
|
CIPRESSA Francesca
(syllabus)
-Introduction: gene structure and organization, coding regions, regulatory sequences, intergenic regions, chromosome and nucleus structure; gene expression: transcription and translation. -From gene to genome: genes mapping and molecular markers; sequencing, genomes annotation and analysis; genome databases; sequences search and alignment. -Organization of complex genomes: protein coding genes; genes for non-coding RNAs; Non-coding RNAs: typology, cellular functions and their influence on gene expression; Repetitive DNA and transposable elements; genetic variability, polymorphisms and mutations; analysis of gene function: gene expression and transcriptome analysis. -Epigenetics and molecular mechanisms underlying epigenetic phenomena. -Molecular genetics methods: DNA cloning; restriction endonuclease; ligase; molecular vectors; transformation and transfection; PCR (Polymerase Chain Reaction); construction and analysis of DNA and cDNA libraries; nucleic acids separation and analysis; Northern blot and Southern blot; array hybridization; in vivo transcripts analysis through in situ hybridization; protein analysis: western blotting; FISH (Fluorescent In Situ Hybridization); SKY (Spectral Karyotype); CGH (Comparative Genomic Hybridization); epigenetic methods: DNA modification with sodium bisulphite, comparative analysis of methylation profiles through microarray, analysis of nuclease hypersensitive sites, ChIp (Chromatin Immunoprecipitation). - Recombinant DNA technology: molecular cloning and manipulation of genetic material for genome editing; generation of transgenic animals; Gene Knock-in, Knock-out and Knock-down; molecular systems for genome editing: CRISPR-Cas9. -Model organisms in advanced genetic analysis: model organisms overview; generation of animal models for genetic diseases through recombinant DNA technology -Applications of molecular genetics: genetic tests in healthcare, clinical diagnostic tests, population screening; pharmacogenetics and personalized medicine; forensic genetics: individual and kinship identification; gene therapy: treatment of genetic diseases and genetic disease therapy; genetic approaches to the treatment of human diseases.
(reference books)
Human molecular genetics, Tom Strachan. In addition, the teacher can provide specific scientific articles and reviews.
|