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Code
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119401 |
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Language
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ITA |
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Type of certificate
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Profit certificate
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| Module:
(objectives)
Modulo di Innovazione tecnologica
Knowledge and understanding: the courseaim to provide the student with the basicprinciples of biotechnologiesapplicable to molecular marker development and use in marker assistedselection and plant breeding in general. Applied knowledge and understanding: basicbiotechnologicalmethodologies are introduced with reference to molecular marker. Making judgements: the advantages and disadvantages of eachpresentedtechnology are illustrated, to develop the student'scriticalsense. Communication skills: students are askedquestionsduring the classes, both to keeptheirattention and to teachthem to askquestions and giveadequateanswers. Learning skills: the theoreticalbases of the differentgeneticbiotechnologiespresented are provided and, for some of them, also the practicalbases, throughlaboratory and in silicoexercises.
Modulo di Colture in vitro dei tessuti vegetali
Knowledge and understanding:Students will gain an in-depthunderstanding of the organization of an in vitro culture lab, necessaryequipment, the fundamentals of sexual and asexualmultiplication, and key concepts of micropropagation, includingrelevantlegislation. Applying knowledge and understanding:Studentswillapplymicropropagation techniques, culture on agar and liquid media, temporary immersion systems, and bioreactors for ex situ conservation and multiplication of temperate fruitspecies. Additionally, theywillimplementrooting and acclimatizationprocedures for micropropagatedmaterial Making judgements:Studentswilldevelop the ability to evaluate the effectiveness of variousmicropropagation techniques and to identify and solve specificissuesrelated to in vitro culture. Communication skills:Studentswillenhancetheircommunication skills by clearlypresenting the results of theirlaboratory activities. Learning skills: The courseencouragescontinuous learning, preparingstudents to stay updated on new techniques and discoveries in the field of micropropagation.
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Language
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ITA |
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Type of certificate
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Profit certificate
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Credits
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3
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Scientific Disciplinary Sector Code
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AGR/03
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Contact Hours
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24
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Type of Activity
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Core compulsory activities
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Teacher
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SILVESTRI Cristian
(syllabus)
Organization of the in vitro culture laboratory: characteristics, structures and planning. Main equipment necessary for the implementation of the micropropagation. Fundamentals of gamic and agamic reproduction of tree, herbaceous and vegetable species of agricultural, ornamental and spontaneous interest. In vitro culture: concepts and definitions. In vitro organogenesis methods and their application. Basics of legislation. In vitro cultures for ex-situ conservation and biodiversity conservation. Cellular totipotency. In vitro micropropagation and multiplication. Somaclonal mutation: causes, consequences, advantages and disadvantages. Culture on agar and liquid media, temporary immersion systems and bioreactors. Mother plants: choice and collection of plant material. Stages of micropropagation and composition: preparation substrates, proliferation, rooting and acclimatization. Application of micropropagation in species of agricultural interest. Recent acquisitions and developments of micropropagation: capsules, synthetic seeds, photoautotrophic cultures. - Practices Practical laboratory activities will be carried out such as: manipulations in aseptic conditions, culture substrates, removal of meristematic apices, stabilization of explants, verification of the in vitro proliferation coefficient obtainable from different types of vitro-derived explants. Implementation of in vitro and in vivo rooting procedures of micropropagated material. Acclimatization and return in situ of the different plant species.
(reference books)
-Detailed reading list will be provided at the beginning of the course. - Pierik R. L. 1999. In Vitro Culture of Higher Plants , Kluwer Academic Publishers - Triggiano et al 2010
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Dates of beginning and end of teaching activities
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From to |
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Delivery mode
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Traditional
At a distance
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Attendance
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not mandatory
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Evaluation methods
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Oral exam
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| Module:
(objectives)
Knowledge and understanding: the courseaim to provide the student with the basicprinciples of biotechnologiesapplicable to molecular marker development and use in marker assistedselection and plant breeding in general. Applied knowledge and understanding: basicbiotechnologicalmethodologies are introduced with reference to molecular marker. Making judgements: the advantages and disadvantages of eachpresentedtechnology are illustrated, to develop the student'scriticalsense. Communication skills: students are askedquestionsduring the classes, both to keeptheirattention and to teachthem to askquestions and giveadequateanswers. Learning skills: the theoreticalbases of the differentgeneticbiotechnologiespresented are provided and, for some of them, also the practicalbases, throughlaboratory and in silicoexercises.
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Language
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ITA |
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Type of certificate
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Profit certificate
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|
Credits
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3
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Scientific Disciplinary Sector Code
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AGR/07
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Contact Hours
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24
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Type of Activity
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Core compulsory activities
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Teacher
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MAZZUCATO Andrea
(syllabus)
Module: Advanced selection techniques Molecular markers and genetic analysis Concept of genetic marker and introduction to the analysis of molecular polymorphism. Biochemical markers. Molecular markers, DNA hybridization and PCR, RFLP markers, RAPD, SCAR, CAPS, AFLP, SSR, and SNP markers. Use of molecular markers for selection Formal genetics and development of genetic maps; use of biparental populations (BC, F2, RIL, DH) and multiparental (MAGIC). Search for markers linked to loci controlling qualitative and quantitative traits; marker assisted selection in plant breeding. Genomic selection. Positional cloning of Mendelian genes and QTLs
(reference books)
Testi di riferimento Barcaccia & Falcinelli - Genetica e genomica Vol. III - Liguori editore. Didactic material from the lecturer
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Dates of beginning and end of teaching activities
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From to |
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Delivery mode
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Traditional
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Attendance
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not mandatory
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Evaluation methods
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Oral exam
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