Teacher
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TIMPERIO Anna Maria
(syllabus)
Gas chromatography-mass spectrometry Instrumentation. Sources: EI, CI, ESI, FAB, MALDI Analyzers: magnetic sector, quadrupole, TOF, ion trap Spectrum interpretation. Acquisition methods: Full Scan and SIM. Qualitative and quantitative analysis by LC-MS.
- HPLC-MS Instrumentation. Electrospray source. Optimization of instrumental parameters.
- Tandem mass spectrometry Triple quadrupole mass spectrometer. Ion trap. Product Ion Scan. Precursor Ion Scan. Neutral Loss Scan. Multiple Reaction Monitoring (MRM). Basic principles of mass spectrometry: The mass spectrum. The high and low resolution molecular peak. Isotope peaks. Measurement of large masses. Fragmentations, rearrangements and adducts. Instrumentation: sample introduction systems; electron impact (EI), chemical ionization (CI), electrospray ionization (ESI), atmospheric pressure chemical ion sources ionization (APCI), matrix assisted laser desorption (MALDI); magnetic ion analyzers, single and triple quadrupole, trap ionic; orbital trap; flight time; detectors. Methods of acquiring and interpreting data in the context of the following experiments: full MS, product ion scan (MS/MS), selected ion monitoring (SIM), extracted ion chromatogram (XIC), multiple reaction monitoring (MRM), neutral loss, precursor ion scan, MSn. Principles, applications and examples of the main techniques hyphenate, including gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS). Analyses quantitative with internal standard and with external standard.
(reference books)
Power point slides shown in class by the teacher will be provided in PDF format. T. Alberio, M. Fasano, P. Roncada "PROTEOMICA" EdiSES;
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