APPLIED BIOLOGY
(objectives)
The teaching is aimed at providing students with a solid and integrated culture in experimental biology and in the various sectors of applied biology and the achievement of equally solid and complete skills in the use of analytical methodologies and advanced methods for the study and understanding of mechanisms molecular of biological processes and for their application in the industrial, health, ecological-evolutionary and biotechnological fields. LEARNING OUTCOMES • Knowledge and understanding: The student will have to demonstrate knowledge of the theoretical and methodological elements of experimental and applied biology, with particular regard to the molecular, biotechnological and biochemical aspects of biological processes, to the knowledge of the interactions between molecules, biomolecules and cells, in normal or altered conditions. • Applying knowledge and understanding: The student will have acquired theoretical and methodological skills that will allow him to apply the correct investigation strategies to solve specific problems in the field of experimental and applied biology. In particular, he / she must be able to interpret and process scientific data derived from the study of scientific articles and databases that use the methodological approaches of experimental and applied biology, also using bioinformatics tools. • Making judgments: Acquisition of independent judgment in reference to the evaluation and interpretation of experimental data reported in scientific articles. • Communication skills: Acquisition of expression skills with appropriate scientific terminology. Acquisition of adequate skills and tools for communication for what concerns the processing and the presentation of data. • Learning skills: Acquisition of adequate skills for the deepening of further skills and knowledge for what concerns the consultation of bibliographic material for continuous updating
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Teacher
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Filippi Silvia -
(syllabus)
Laboratory safety and laboratory set-up. The Scientific Method: How to design a biological-molecular experiment and how to obtain results Model organisms in experimental and applied biology both in vitro and in vivo. In vitro models "the cell lines": set-up, propagation and storage of suspension and substrate-adherent cultures. Determination and control of contamination of cell cultures by microorganisms. Primary (tissue explants) and immortalized cultures. 2D and 3D and induced pluripotent (IPS) cell cultures. Application of cell lines in the biological field: assays of cell viability and cell proliferation. Cytotoxicity and IC50 calculation In vivo models: The biology of C. Elegans and Drosophila Melanogaster: applications in biology (miRNA and aging studies) D. Rhenium (Zebrafish) application in neuroscience X.Laevis application in embryogenesis M.Musculus application in the study of aging-related diseases Bacteria and bacteriophages application in biotechnology: nanoantibodies. Molecular basis of regulatory mechanisms of adhesion, cell motility and cytoskeletal reorganization. Methods for the study of such molecular mechanisms. Stem cells and migration. Main techniques for the study of the cell cycle, aging processes, and the study of tumorigenesis. Light, fluorescence, transmission and scanning microscopy: principles and application Gene silencing and overexpression: Why change the expression levels of a gene? Therapeutic applications of silencing and overexpression. CRISPR technique, Antisense Oligonucleotides (ASOs).
LABORATORY EXERCISE: Cell cultures maintenance and propagation Viability and apoptosis assays; IC50 calculation and cytotoxicity verification Confocal microscopy: slide preparation and analysis.
(reference books)
Materiale fornito dal Docente durante le lezioni
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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not mandatory
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Evaluation methods
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Oral exam
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