Teacher
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GEVI Federica
(syllabus)
Nutritional Clinical Biochemistry Program: Energy metabolism and related pathologies Fundamentals of laboratory medicine; Biochemical-Clinical Analysis with particular reference to the alterations of the biochemical picture that can be seen in the pathologies of the organs and systems Analytical techniques in clinical biochemistry and laboratory medicine Carbohydrate metabolism. Digestion and absorption of carbohydrates. Synthesis and demolition of glycogen. Glucose catabolism and anabolism. Regulatory mechanisms of glucose metabolism Lipid metabolism. Digestion and absorption. Catabolism and anabolism of fatty acids. Ketogenesis. Adjustments of lipid metabolism. Notes on the metabolism of phospholipids and steroids. Obesity in particular in the pediatric age. Protein metabolism. Digestion and absorption. Enzymatic hydrolysis of proteins. Mechanism of action of some peptidases. Amino acid catabolism. Ureogenesis. Purine catabolism. Adjustments of amino acid metabolism. Haem catabolism. Protein synthesis. Citric acid cycle (Krebs cycle). Biological and bioenergetic oxidations. Energy significance of oxidative reactions. The redox potential. The mitochondrion: shuttle mechanisms for the transport of electrons, the respiratory chain Metabolic Diseases subject to Extended Neonatal Screening. Organic Acidurias, Beta oxidation defects, amino acid metabolism defects, urea cycle defects, diagnosis, confirmation and therapy diagnostic tests. Second tier test. Basic statistical tools. Analytical performance evaluation (Precision, Accuracy, LOD, LOQ), Diagnostic Tests and ROC Curves. Brain-to-Brain loop process. Pre-anil phase, biological variability, sample variability, quality indicators, main sources of error. Blood: Arterial, Venous and Capillary. Blood gas analysis, pathophysiology, oxygenation, Acid-Base balance. Hematological diagnostics: Red (RBC), white (WBC) cells and platelets. Blood smear, Hematocrit (Hct), Hemoglobin (Hgb), Mean Corpuscular Volume (MCV), Mean Corpuscular Concentration (MCHC), Red Cell Amplitude Distribution (RDW). Platelet Indices: Mean platelet volume (MPV), mean corpuscular diameter (PDW) and Plateletcritus (PCT). LDL receptor, LPL. Lipoprotein metabolism, exogenous and endogenous way. Dyslipidemias, Fredrickson's classification, Pathogenetic classification. Lipid profile and diagnosis. Enzymatic Determinations in Diagnostics. Plasma concentration of enzymes (Release and Clearance). Muscle, Hepatic, Pancreatic, Bone and Cardiac Enzymes. Techniques for measuring the enzymatic activity of the main enzymes of diagnostic interest. Serum protein electrophoresis. Electrophoretic techniques and application to plasma cell dyscrasias. The Kidney, urine formation, laboratory diagnostics, biomarkers of kidney diseases, acid-base balance, classification of kidney diseases. Chemical-physical and morphological examination of urine Diabetes. Blood sugar, diabetes classification, diagnosis and complications Diabetes in pregnancy
(reference books)
Biochimica clinica e medicina di Laboratorio III edizione Marcello Ciaccio, Giuseppe Lippi Edises editrice. Biochimica della nutrizione Leuzzi, Bellocco, Barreca Zanichelli
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