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Teacher
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KUZMINSKY Elena
(syllabus)
The class and lab. activities lectures will be focused on the following groups of topics/abilities: methods available for in vitro propagation, variation in cultures and plants, the control of contaminants and diseases, genetic, environmental and tissue dependent factors affecting growth and morphogenesis in cultures, the nature and uses of plant growth regulators, and the components, preparation and uses of culture media. Special problems encountered in initiating and maintaining cultures, how shoots and plantlets are converted into plants and established in the external environment, how performance in greenhouse and field can be altered, commercial aspects and economics of production, a tabulation of recent patents, and tables of published work on plant micropropagation of a large variety of trees and plants.
1) Plant Tissue Culture Techniques.
2) Woody Plant Propagation and Micropropagation.
3) Variation in Cultures and Regenerated Plants.
4) Equipment and Procedures.
5) Controlling Persistent Contaminants and Plant Diseases.
6) Storing and Distributing Clonal Material.
7) Factors Affecting Growth and Morphogenesis of woody plants (I. Genotype and the Physical Environment, II. Tissue Dependent Factors.
8) The Components of Culture Media.
9) The Derivation, Preparation and Use of Plant Tissue Culture Media.
10) Plant Growth Regulators
11) Appropriate Growth Factors and Media for woody plants.
12) Problems in Initiating and Maintaining Cultures, especially in woody plants.
13) Rooting and Establishment.
14) The Phenotype of micropropagated material.
15) Commercial Micropropagation.
16) Micropropagation in Practice
(reference books)
1. Plant Cell Culture, essential methods (2010). Edited by M.R. Davey and P. Anthony. Wiley-Blackwell.
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