Teacher
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PICCHIETTI Simona
(syllabus)
Light microscopy: principles and applications. The cyto-histological techniques as a diagnostic method. Histochemical methods. Laser microdissection microscope: from its principle to applications in various research areas. Immunohistochemistry and immunofluorescence: applications in the biological field and applied biomedical research. Antibodies and immunostaining. Fluorescence and fluorochromes. Fluorescent proteins. Fluorescence microscopy and Confocal laser scanning microscopy: guiding principles of specimen preservation. Two-photon Laser Scanning Microscopy. Super resolution microscopy. In situ hybridization: general principles and applications. The Transmission and Scanning electron microscopes. The value of the electron microscopy in diagnosis. SEM-EDS elemental microanalysis. The Environmental Scanning Electron microscope and its applications. Immunogold labelling and applications in the biomedical field. Microscopy for nanotechnology. Atomic Force Microscopy: applications in pharmaceutical research. Discussion of scientific papers. Description of the hands-on activities. Histological sample preparation for light microscopy: fixation, embedding, sectioning and staining. Examination of specimens under the light microscope. Immunohistochemical methods: the ABC immunoperoxidase technique. Sample preparation for fluorescence microscopy. Examination of specimens under the fluorescence microscope and confocal laser scanning microscope. Laser microdissection of biological samples. Sample processing for transmission electron microscopy (TEM): fixation, dehydration, resin embedding, thin sectioning, positive staining. Negative staining of biological samples. TEM analysis. The immunogold labelling: sample processing. The scanning electron microscope and SEM-EDS elemental microanalysis: processing and examination of samples. The hands-on activities will be carried out at the Zoology and CGA laboratories.
(reference books)
Scientific papers and slides of the lessons. https://www.unitus.it/it/unitus/Studenti/articolo/moodle
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