Teacher
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RINALDUCCI Sara
(syllabus)
SIGNAL TRANSDUCTION: PATHWAYS REGULATING GENE EXPRESSION. Single-pass transmembrane receptors. Tyr-kinases. Adaptor proteins. Protein interaction domains (SH2, SH3, PTB, WW, PH, PDZ etc.). Transduction pathways from the membrane to the nucleus: Src, Ras, MAPKs, PI-3K proteins. TGF-beta/Smad signaling. Cytokine receptors and JAK/STAT pathway. Cell surface receptors with multiple membrane spanning segments. CREB activation, PKA and AMPc. Wnt/beta-catenin signaling. NFkB.
CONTROL OF GENE EXPRESSION. Details on gene regulation in bacteriophage lambda. Nucleosomes, histones and post-translational modifications (HATs, HDACs, HKMTs, PRMTs). Bromodomains, Chromodomains, PHD finger and TUDOR domains. Chromatin remodeling complexes. Repressors, activators: structural domains. The yeast GAL regulon. Sir proteins. Combinatorial control of the mating-type genes from yeast. HMG, nuclear non-histone proteins. IFN-beta gene and assembly of the enhanceosome. Molecular biology of HIV.
CATALYTIC AND REGULATORY RNAs. Introns of type I and II. Ribozymes and Riboswitches. RNA interference. siRNAs. The biogenesis and function of microRNAs. Drosha, Pasha, Dicer, the Argonaute proteins, RISC. rasiRNAs. Gene silencing, RITS. mirMASA technology.
(reference books)
Advised books: Biologia Molecolare della Cellula, Lodish et al., 2009-Zanichelli. Biologia Molecolare del Gene, Watson J.D. et al., sesta edizione, 2009-Zanichelli Geni e Segnali, Ptashne-Gann, 2004-Zanichelli Regolazione genica, Ptashne, 2006-Zanichelli Il Gene, Lewin et al., 2011-Zanichelli.
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