Teacher
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CARUSO Carla
(syllabus)
Proteic engineering fundamentals (2 CFU) Recombinant protein expression in prokaryotic systems: principles and applications. Recombinant protein expression in eukaryotic systems: -Expression in Saccharomyces cerevisiae and Pichia pastoris; -Expression in plants.
Experimental laboratory (1 CFU) • Enzymatic digestion of pGEM-HEL plasmid and purification of the HEL gene from agarose gel; • Subcloning of the HEL gene in the expression vector pGEX-4T; • Transformation of competent BL-21 cells with the newly generated GST-HEL plasmid; • Expression of recombinant GST-HEL in BL-21 cells and analysis of the recombinant protein trough SDS-PAGE.
The cell cycle (3 CFU) General strategy and cell cycle phases (M, G1(G0), S and G2. Experimental systems for the study of the cell cycle: Xenopus leavis eggs, mammalian cells and yeast. Molecular regulation of MPF and SPF: mitotic and G1 cyclins. Cdks and cyclins in mammalian cell cycle. Cell cycle checkpoints. Hereditary and sporadic retinoblastoma and role of Rb in cell cycle regulation. UV-damaged DNA and role of p53. Oncogenes and oncoproteines.
(reference books)
Cell Cycle
Murray A & Hunt T, The cell cycle, an introduction, Oxford University Press, New York. Alberts B, Johnson A, Lewis J, Morgan D, Raff M, Roberts K & Walter P, Biologia Molecolare della Cellula, Zanichelli, 2016 (VI Edizione) Harvey Lodish, A Berk, C.A. Kaiser, M. Krieger, M.P. Scott, A. Bretscher, P. Ploegh, Paul Matsudaira Biologia Molecolare della Cellula, Zanichelli, 2009 (III edizione)
Protein engineering
Glick & Pasternak, Biotecnologia Molecolare, Zanichelli Primrose, Twyman & Old, Ingegneria Genetica, Zanichelli Watson, Caudy, Myers & Witkowski, DNA Ricombinante, Zanichelli 2008 (II Edizione) Brown, Biotecnologie molecolari, Zanichelli 2007
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