Teacher
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BARGHINI Paolo
(syllabus)
History of microbiology: discovery of the microbial world; the “Abiogeny” dispute, microorganisms and their environment, impact of microorganisms on man. Citology: prokaryotic and eukaryotic cells: general concepts, structure and ultra-structure of bacterial cells, structure and function of cytoplasmic membrane, transports through the cytoplasmic membrane, cell wall, structure and function, the cell wall of Gram + and Gram – bacteria, Archaeal and eukaryotic cell walls, capsules and other envelopes, motility, flagella and chemotaxis, endospores structure and function. Cell physiology: reminders of chemistry and cell biochemistry (redox reactions, hydrogen and electron transport, high energy compounds), biological energy production, glycolysis and alternative pathways; NAD re-oxidation: fermentations (alcoholic and lactic); aerobic respiration (TCA, electron transport phosphorylation, energy balance in respiration), mentions of anaerobic respiration and biosynthesis. Microbial growth: growth of a single cell and of a microbial population, diauxic growth, effect of environmental parameters on growth (pH, temperature, etc.), methods for the control of microbial growth. Microbial genetic: mentions to molecular genetics (replication, transcription, translation, genetic code and mutations, etc.), regulation of protein synthesis and activity, genetic elements, bacterial recombination. Mention of microbial ecology and environmental biotechnology: isolation and identification of environmental microorganisms, principal biogeochemical cycles (Carbon, Nitrogen, etc.), interaction between microorganisms and other organisms, role of microorganisms in environmental decontamination, aerobic/anaerobic catabolism of environmental pollutants, treatment of water and wastewater. Basic microbiology laboratory techniques. The microscope and mentions of optical physics, preparation of microscope slides, the electron microscope, the pure culture, mention of microbial nutrition, preparation of culture media, theory and practice of sterilization, measure of microbial growth.
(reference books)
Dehò Gianni e Galli Enrica: Biologia dei microrganismi – III edizione. Casa Editrice Ambrosiana (2018). Brock Biologia dei microorganismi: microbiologia generale, ambientale e industriale. 14° edizione (2016). Casa Editrice Ambrosiana.
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