Ruggiero Paolo

Docente a contratto esterno 
Ateneo Università degli Studi della TUSCIA 
Struttura di afferenza Dipartimento di DIBAF - Dipartimento per la Innovazione nei Sistemi Biologici, Agroalimentari e Forestali 

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PAOLO RUGGIERO Curriculum vitae EDUCATION High School: Liceo Classico, Viterbo, Italy, 1973 University: Laurea (Master of Science) in Biological Sciences with honours, University of Siena, Italy, 1978 (Thesis supervisor: Prof. Vitaliano Pallini) Languages: Italian (mother tongue), English, French CAREER HISTORY January – June 1979: Institute of Pharmacology, University of Siena, Italy. July 1979 – September 1980: military service. October 1980 – March 1982: Teacher of Sciences at High Schools, Viterbo, Italy. April 1982 – September 1990: Scientist, Sclavo SpA, Research Center, Siena, Italy. Studies on cytoskeleton: protein purification, computer design of synthetic peptides to generate specific antibodies, immunohistochemistry. Studies on Papilloma virus: immunohistochemistry, in situ DNA hybridization. Participation in phase I clinical trial of recombinant pertussis vaccine: protein purification, human serology. Within this period: March - June 1984: Developmental Biology Unit, University of Bielefeld, Germany, guest of Prof. Brigitte Jockusch; January - March 1988: Pathology Dept., C.M.U., University of Geneva, Switzerland, guest of prof. Giulio Gabbiani. October 1990 – March 2000: Dompé SpA, Research Center, L'Aquila, Italy: (1990 - 1996) Dept. of Biotechnology: Head, Laboratory of Biochemistry (including a 10-liter scale Fermentation and Purification Pilot Plant). (1997 - 1999) Dept. of Chemistry and Fermentation: Head, Laboratory of Fermentation and Downstream. (1999 - 2000): Dept. of Biotechnology: Head, Laboratory of Protein Science and Fermentation (including a 300-liter scale Pilot Plant). Start-up and management of laboratories, start-up and first phases of validation (IQ, OQ) and management of Pilot Plants. Studies on interleukin-1 family and receptors. Fermentation, purification and characterization of recombinant cytokines. Member of the working groups of Farmindustria (Italian Association of Pharmaceutical Companies) 1) Rules and guidelines about genetically modified microorganisms, and 2) Intellectual property rights of biotechnological products. April 2000 – May 2006: Chiron srl, Research Center, Siena, Italy: (2000 - 2001) Immunology: Senior Scientist. (2002 - 2006) Serology and Animal Models: Senior Scientist. Set up and/or optimization of animal models of bacterial infection: Helicobacter pylori, Streptococcus pneumoniae. Studies on pathological mechanisms in vitro and in vivo. Evaluation of immune response and investigational vaccines efficacy in animal models. Designing and managing of experimental studies. Coordination of external pre-clinical studies on Helicobacter pylori vaccine efficacy. Whitin this period: February 2003: Stanford University, U.S., CA, guest of Prof. Stanley Falcow. June 2006 – February 2015: Novartis Vaccines and Diagnostics srl, Research Center, Siena, Italy: (June 2006 - May 2008) Translational Medicine: Senior Scientist (main activities as above). (May 2008 – February 2015) Translational Medicine: Head of Investigative Pathology. Set up and management of the laboratories of histopathology. Designing and managing of experimental studies. Contribution to setup of animal models. Management of histopathological evaluation of animal models of infection and vaccine efficacy. Studies on local response to infection and vaccination. Coordination of external collaborations for histopathological analyses. Activities done in particular for the Staphylococcus aureus and Group B Streptococcus vaccine projects. March 2015 – June 2016: GlaxoSmithKleine (GSK) vaccines srl, Research & Development, Siena, Italy: (March 2015 – January 2016) Translational Medicine: Head of Investigative Pathology (main activities as above) (February 2016 – June 2016) GlaxoSmithKleine (GSK) vaccines srl, Preclinical Research & Development, Siena, Italy: Preclinical Evidence & Bioassays: Senior Scientist. Management of histopathological analyses in the frame of non-clinical studies on immunogenicity and efficacy of investigational vaccines, and on effects and mechanisms of vaccine adjuvants. Designing and managing of experimental studies. Presently (academic year 2018-2019): Contract Professor (Principles of Pathology and Biopharmaceutics), University of Tuscia, DIBAF, Viterbo, Italy. PUBLICATIONS (research papers, reviews, and book chapters) 1. P Di Simplicio, M Palmi, P Ruggiero, G Segre: Contenuto di glutatione-S-transferasi e di glutatione nel fegato e nel sangue di ratti intossicati con tioacetamide. (Glutathione-S-transferase and glutathione in the liver and blood of rats poisoned by thioacetamide). Boll. Soc. Ital. Biol. Sper. 1980 LVI:795-801 (Italian language) [PMID: 7448078] scopus 2-s2.0-0019335679 2. V Pallini, C Mencarelli, L Bracci, M Contorni, P Ruggiero, A Tiezzi, R Manetti: Cytoplasmic nucleoside triphosphatases similar to axonemal dynein occur widely in different cell types. J. Submicrosc. Cytol. 1983 15:229-235 [PMID: 6133010] scopus 2-s2.0-0020667591 3. P Ruggiero, A Tiezzi, M Bugnoli, V Pallini: Immunoreactive analogues of brain microtubule-associated protein 2 (MAP2) in mammalian kidney and liver. J. Submicrosc. Cytol. 1984 16:131-132 4. D Armellini, A Massone, A Tiezzi, P Leoncini, P Ruggiero, M Bugnoli, G Scapigliati, V Pallini: Monoclonal antibodies: development in immunocytochemistry. In: New Technologies in Clinical Laboratory Science, MTP Press Ltd. 1986 97-105 ISBN 978-94-009-4928-7 5. C Mencarelli, A Moscatelli, V Pallini, A Tiezzi, M Contorni, P Ruggiero, A Massone: Monoclonal Antibodies to the Ciliary axoneme: tools for biochemical dissection and comparative studies. In: Biology of Reproduction and Cell Motility in Plants and Animals, M Cresti and R Dallai Editors, University of Siena 1986 75-105 6. C Mencarelli, M Bugnoli, M Contorni, A Moscatelli, P Ruggiero, V Pallini: Phosphorilated epitopes of neurofilaments have been conserved during chordate evolution. Biochem. Biophys. Res. Commun. 1987 149:807-814 [PMID: 2447884] scopus 2-s2.0-0023554063 7. P Ruggiero, E Bellizzi De Marco, R Petracca, P Leoncini: Immunolocalization of bovine keratin n.22 using antibodies to synthetic peptides. In: Structure and Functions of the Cytoskeleton, BAF Rousset Editor, Colloque INSERM/John Libbey Eurotext Ldt. 1988 171:25-31 [ISBN: 2855983517 (INSERM), 0861961498 (J. Libbey)] 8. MG Ricci, M Cintorino, G Morgante, P Ruggiero, C Marsiglietti, PL Leone, E Turillazzi, R La Rosa, S Danero: Condilomatosi genitale a localizzazione multipla. Diagnosi di tipo virale mediante ibridazione in situ (Genital condylomatosis with multiple localization. Viral diagnosis by in situ hybridization). Pat. Clin. Ost. Gin. 1989 XVII:74-77 9. P Leoncini, R Petracca, P Ruggiero, M Cintorino, S Syrjanen, R Mantyjarvi, K Syrjanen: Expression of cytokeratin n.19 polypeptide in genital Papillomavirus lesions. Gynecol. Obstet. Invest. 1990 29:59-66 [PMID: 1693587] scopus 2-s2.0-0025055430 10. P Ruggiero, R Petracca, P Leoncini: Immunolocalization of intermediate filament proteins using antibodies to synthetic peptides. J. Histochem. Cytochem. 1990 38:993-999 [PMID: 1693936] scopus 2-s2.0-0025322940 11. A Podda, L Nencioni, MT De Magistris, A Di Tommaso, P Bossù, S Nuti, P Pileri, S Peppoloni, M Bugnoli, P Ruggiero, I Marsili, A D'Errico, A Tagliabue, R Rappuoli: Metabolic, Humoral and Cellular Responses in Adult Volunteers Immunized with the Genetically Inactivated Pertussis Toxin Mutant PT-9K/129G. J. Exp. Med. 1990 172:861-868 [PMID: 2201750] scopus 2-s2.0-0025050450 12. M Pizza, A Covacci, A Bartoloni, M Perugini, L Nencioni, R Manetti, M Bugnoli, P Ruggiero, R Olivieri, T De Magistris, R Rappuoli: Genetic Detoxification of Pertussis Toxin and Development of a new Vaccine against Pertussis. Biotech Forum Europe 1990 7:385-386 13. P Tosi, V Pallini, M Cintorino, M Bugnoli, A Covacci, R Petracca, S Syrjanen, MT Del Vecchio, R Mantyjarvi, P Ruggiero, D Armellini, F Chang, K Syrjanen: Use of antibodies against a synthetic peptide of the E6 protein of Human Papillomavirus (HPV) type 16 for the diagnosis of genital HPV lesions. Cytopathology 1993 4:3-15 [PMID: 8384016] scopus 2-s2.0-0027411350 14. R Bertini, C Bizzarri, C D'Ettorre, G Bertolasi, P Ruggiero, A Tagliabue, D Boraschi: New insights in chemokine interaction. Int. J. Immunopathol. Pharmacol. 1994 7:221-225 15. C Mencarelli, A Tiezzi, P Ruggiero, M Contorni, A Massone, A Moscatelli, V Pallini. Heterogeneous localization of epitopes along axonemes of mammalian cilia. Biol. Cell. 1995 83:179-84. [PMID: 7549913] scopus 2-s2.0-0029085764 16. R Bertini, W Luini, S Sozzani, B Bottazzi, P Ruggiero, D Boraschi, D Saggioro, L Chieco-Bianchi, P Proost, J Van Damme, A Mantovani: Identification of MIP-1alpha/LD78 as a monocyte chemoattractant released by the HTLV-I transformed cell line MT4. AIDS Res. Hum. Retroviruses 1995 11:155-160 [PMID: 7537510] scopus 2-s2.0-0028988567 17. L Cilenti, E Toniato, P Ruggiero, C Fusco, AR Farina, A Tiberio, AC Hayday, A Gulino, L Frati, S Martinotti: Transcriptional modulation of the human intercellular adhesion molecule gene I (ICAM-I) by retinoic acid on melanoma cells. Exp. Cell Res. 1995 218:263-270 [PMID: 7737364] scopus 2-s2.0-0029067509 18. G Maurizi, P Bossù, D Boraschi, A Tagliabue, P Ruggiero: Sporulation: an alternative way to recover recombinant proteins from B. subtilis. Biotechnol. Bioeng. 1995 48:197-200 scopus 2-s2.0-0029637087 19. P Bossù, U Visconti, P Ruggiero, G Macchia, M Muda, R Bertini, C Bizzarri, A Colagrande, V Sabbatini, G Maurizi, E Del Grosso, A Tagliabue, D Boraschi: Transfected type II IL-1 receptor impairs responsiveness of human keratinocytes to IL-1. Am. J. Pathol. 1995 147:1852-1861 [PMID: 7495308] scopus 2-s2.0-0028789085 20. D Boraschi, P Bossù, P Ruggiero, A Tagliabue, R Bertini, G Macchia, C Gasbarro, L Pellegrini, G Melillo, E Ulisse, U Visconti, C Bizzarri, E Del Grosso, AR Mackay, G Frascotti, F Frigerio, R Grifantini, G Grandi: Mapping of receptor binding sites on IL-1beta by reconstruction of IL-1ra-like domains. J. Immunol. 1995 155:4719-4725 [PMID: 7594472] scopus 2-s2.0-0028788688 21. D Boraschi, P Bossù, G Macchia, P Ruggiero, A Tagliabue: Structure-function relationship in the IL-1 family. Front. Biosci. 1996 1:270-308 ( [PMID: 9159234] scopus 2-s2.0-0001031383 22. G Maurizi, V Di Cioccio, G. Macchia, P Bossù, C Bizzarri, U Visconti, D Boraschi, A Tagliabue, P Ruggiero: Purification of human recombinant interleukin 1 receptor antagonist proteins upon Bacillus subtilis sporulation. Protein Expr. Purif. 1997 9:219-227 [PMID: 9056487] scopus 2-s2.0-0031105133 23. P Ruggiero, P Bossù, G Macchia, E Del Grosso, V Sabbatini, R Bertini, A Colagrande, C Bizzarri, G Maurizi, V Di Cioccio, G D'Andrea, A Di Giulio, G Grandi, A Tagliabue, D Boraschi: Inhibitory activity of IL-1ra depends on the balance between binding capacity for IL-1RI and IL-1RII. J. Immunol. 1997 158:3881-3887 [PMID: 9103457] scopus 2-s2.0-0031569584 24. P Bossù, P Ruggiero, G Macchia, G Maurizi, C Bizzarri, D Neumann, A. Tagliabue, D Boraschi: Interaction between IL-1 and CNTF in the regulation of neuroblastoma cell functions. Eur. Cytokine Netw. 1997 8:367-374 [PMID: 9459616] scopus 2-s2.0-0031463462 25. P Bossù, M. Vanoni, V Wanke, MP Cesaroni, F Tropea, G Melillo, C Asti, S Porzio, P Ruggiero, V. Di Cioccio, G Maurizi, A Ciabini, L Alberghina: A dominant negative Ras-specific guanine nucleotide exchange factor reverses neoplastic phenotype in k-Ras transformed mouse fibroblasts. Oncogene 2000 19:2147-2154 [PMID: 10815806] scopus 2-s2.0-0034690029 26. G Maurizi, A Ciabini, V Di Cioccio, P Ruggiero: Peroxidase-active molecular weight markers for direct detection in western blot. Anal. Biochem. 2000 286:295-296 [PMID: 11067753] scopus 2-s2.0-0034669273 27. P Bossù, E. Del Grosso, MP Cesaroni, G Maurizi, N Balestro, A Stoppacciaro, E Del Giudice, P Ruggiero, D Boraschi: Balance between autocrine interleukin-1beta and caspases defines life versus death of polymorphonuclear cells. Eur. Cytokine Netw. 2001 12:177-186 [PMID: 11282563] scopus 2-s2.0-0035074526 28. E Del Giudice, A Ciaramella, N Balestro, D Neumann, PG Romano, MP Cesaroni, G Maurizi, P Ruggiero, D Boraschi, P Bossù: Neutrophil apoptosis in autoimmune Fas-defective MRL lpr/lpr mice. Eur. Cytokine Netw. 2001 12:510-517 [PMID: 11566632] scopus 2-s2.0-0034807901 29. P Ruggiero, S Peppoloni, D Berti, R Rappuoli, G Del Giudice: New strategies for the prevention and treatment of Helicobacter pylori infection. Expert Opin. Investig. Drugs 2002 11:1127-1138 [PMID: 12150706] scopus 2-s2.0-0036049744 30. S Peppoloni, S Mancianti, G Volpini, S Nuti, P Ruggiero, R Rappuoli, E Blasi, G Del Giudice: Antibody-dependent macrophage-mediated activity against Helicobacter pylori in the absence of complement. Eur. J. Immunol. 2002 32:2721-2725 [PMID: 12355423] scopus 2-s2.0-0036773534 31. F Tombola, S Campello, L De Luca, P Ruggiero, G Del Giudice, E Papini, M Zoratti: Plant polyphenols inhibit VacA, a toxin secreted by the gastric pathogen Helicobacter pylori. FEBS Lett. 2003 543:184-189 [PMID: 12753930] scopus 2-s2.0-0037716898 32. P Ruggiero, S Peppoloni, R Rappuoli, G Del Giudice. The quest for a vaccine against Helicobacter pylori: how to move from mouse to man? Microbes Infect. 2003, 5:749-756 [PMID: 12814776] scopus 2-s2.0-0037565075 33. S Peppoloni, P Ruggiero, M Contorni, M Morandi, M Pizza, R Rappuoli, A Podda, G Del Giudice: Mutants of the Escherichia coli heat-labile enterotoxin as safe and strong adjuvants for intranasal delivery of vaccines. Expert Rev. Vaccines 2003 2:285–293 [PMID: 12899578] scopus 2-s2.0-0037992894 34. P Ruggiero, S Flati, V Di Cioccio, G. Maurizi, G. Macchia, A Facchin, R Anacardio, A Maras, M Lucarelli, D Boraschi: Glycosylation enhances functional stability of the chemotactic cytokine CCL2. Eur. Cytokine Netw. 2003 14:91-96 [PMID: 12957789] scopus 2-s2.0-10744229539 35. M Vajdy, M Singh, M Ugozzoli, M Briones, E Soenawan, L Cuadra, J Kazzaz, P Ruggiero, S Peppoloni, F Norelli, G Del Giudice, D O’Hagan: Enhanced mucosal and systemic immune responses to Helicobacter pylori antigens through mucosal followed by systemic immunizations. Immunology 2003 110:86-94 [PMID: 12941145] scopus 2-s2.0-0041817682 36. S Ricci, G Macchia, P Ruggiero, T Maggi, P Bossù, L Xu, D Medaglini, A Tagliabue, L Hammarström, G Pozzi, D Boraschi: In vivo mucosal delivery of bioactive human interleukin 1 receptor antagonist produced by Streptococcus gordonii. BMC Biotechnol. 2003 3:15 ( [PMID: 13129437] scopus 2-s2.0-0642303861 37. P Bossù, D Neumann, E Del Giudice, A Ciaramella, I Gloaguen, G Fantuzzi, CA Dinarello, E Di Carlo, P Musiani, PL Meroni, G Caselli, P Ruggiero, D Boraschi: IL-18 cDNA vaccination protects mice from spontaneous lupus-like autoimmune disease. Proc. Natl. Acad. Sci. USA 2003 100:14181-14186 [PMID: 14615579] scopus 2-s2.0-10744222448 38. JD Dubreuil, P Ruggiero, R Rappuoli, G Del Giudice: Effect of heparin binding on Helicobacter pylori resistance to serum. J. Med. Microbiol. 2004 53:9-12 [PMID: 14663099] scopus 2-s2.0-1542741013 39. G Rossi, S Romagnoli, L Lauretti, L Pancotto, E Taccini, R Rappuoli, G Del Giudice, P Ruggiero: Helicobacter pylori infection negatively influences pregnancy outcome in a mouse model. Helicobacter 2004 9:152-157 [PMID: 15068417] scopus 2-s2.0-1942440332 40. P Ruggiero, R Rappuoli, G Del Giudice: Helicobacter pylori. In: Novel Vaccination Strategies, SHE Kaufmann Editor, Wiley-VCH 2004 435-462 [ISBN: 9783527305230 (print), 9783527601448 (online); DOI: 10.1002/3527601449] 41. G Rossi*, P Ruggiero*, S Peppoloni, L Pancotto, D Fortuna, L Lauretti, G Volpini, S Mancianti, M Corazza, E Taccini, F Di Pisa, R Rappuoli, G Del Giudice: Therapeutic vaccination against Helicobacter pylori in the beagle dog experimental model: safety, immunogenicity and efficacy. Infect. Immun. 2004 72:3252-3259 [*equal contribution] [PMID: 15155627] scopus 2-s2.0-2542573391 42. P Ruggiero, A Alloueche, R Rappuoli, G Del Giudice: Models for bacterial infectious diseases: Helicobacter pylori. Drug Discovery Today: Disease Models 2004 1:95-100 [] scopus 2-s2.0-23744456877 43. S Porzio, P Bossù, P Ruggiero, D Boraschi, A Tagliabue: Mucosal delivery of anti-inflammatory IL-1Ra by sporulating recombinant bacteria. BMC Biotechnol. 2004 4:27 ( [PMID: 15516267] scopus 2-s2.0-13244252314 44. M De Bernard, A Cappon, L Pancotto, P Ruggiero, G Del Giudice, C Montecucco: The Helicobacter pylori VacA cytotoxin activates RBL-2H3 cells by inducing cytosolic calcium oscillations. Cell. Microbiol. 2005 7:191-198 [PMID: 15659063] scopus 2-s2.0-13844315698 45. P Ruggiero, F Tombola, G Rossi, L Pancotto, L Lauretti, G Del Giudice, M Zoratti. Polyphenols reduce gastritis induced by Helicobacter pylori infection or VacA toxin administration in mice. Antimicrobial Agents and Chemotherapy 2006 50:2550-2552 [PMID: 16801443] scopus 2-s2.0-33745628100 46. M Hilleringmann, W Pansegrau, M Doyle, S Kaufman, ML Mackichan, C Gianfaldoni, P Ruggiero, A Covacci. Inhibitors of Helicobacter pylori ATPase Cag-alpha block CagA transport and cag virulence. Microbiology 2006 152:2919-2930 [PMID: 17005973] scopus 2-s2.0-33749593502 47. P Ruggiero, G Rossi, F Tombola, L Pancotto, L Lauretti, G Del Giudice, M Zoratti. Red wine and green tea reduce H. pylori- or VacA-induced gastritis in a mouse model. World J. Gastroenterol. 2007 13:349-354 [PMID: 17230601] scopus 2-s2.0-33846590546 48. C Gianfaldoni, S Censini, M Hilleringmann, M Moschioni, C Facciotti, W Pansegrau, V Masignani, A Covacci, R Rappuoli, MA Barocchi, P Ruggiero. Streptococcus pneumoniae pilus subunits protect mice against lethal challenge. Infect. Immun. 2007 75:1059-1062 [PMID: 17145945] scopus 2-s2.0-33846847480 49. P Ruggiero. Helicobacter pylori pathogenesis and vaccines. In: Immunity against mucosal pathogens, M. Vajdy Editor, Springer 2008 195-218 [ISBN: 978-1-4020-8411-9 (print), 978-1-4020-8412-6 (online)] scopus 2-s2.0-84919708696 50. JF Romero, A Ciabattini, P Guillaume, G Frank, P Ruggiero, E Pettini, G Del Giudice, D Medaglini, G Corradin. Intranasal administration of the synthetic polypeptide from the C-terminus of the circumsporozoite protein of Plasmodium berghei with the modified heat-labile toxin of Escherichia coli (LTK63) induces a complete protection against malaria challenge. Vaccine 2009 27:1266-1271 [PMID: 19111883] scopus 2-s2.0-58749088034 51. DR Negri, D Pinto, S Vendetti, A Riccomi, P Ruggiero, G Del Giudice, MT De Magistris. Cholera toxin and E. coli heat-labile enterotoxin, but not their non-toxic counterparts, improve the antigen presenting cell function of human B lymphocytes. Infection and Immunity 2009, 77:1924-1935 [PMID: 19223474] scopus 2-s2.0-65449133249 52. C Gianfaldoni, S Maccari, L Pancotto, G Rossi, M Hilleringmann, W Pansegrau, A Sinisi, M Moschioni, V Masignani, R Rappuoli, G Del Giudice, P Ruggiero. Sortase A confers protection against Streptococcus pneumoniae in mice. Infect. Immun. 2009 77:2957-2961 [PMID: 19433540] scopus 2-s2.0-67650064656 53. DR Negri, A Riccomi, D Pinto, S Vendetti, A Rossi, R Cicconi, P Ruggiero, G Del Giudice, MT De Magistris. Persistence of mucosal and systemic immune responses following sublingual immunization. Vaccine 2010, 28:4175-4180 [PMID: 20412876] scopus 2-s2.0-77952671933 54. M Moschioni, C Emolo, M Biagini, S Maccari, W Pansegrau, C Donati, M Hilleringmann, I Ferlenghi, P Ruggiero, A Sinisi, MG Pizza, N Norais, M Barocchi, V Masignani. The two variants of Streptococcus pneumoniae pilus-1 RrgA adhesin retain the same function and elicit cross-protection in vivo. Infect. Immun. 2010, 78:5033-5042 [PMID: 20823200] scopus 2-s2.0-78649922984 55. P Ruggiero: Helicobacter pylori and inflammation. Current Pharmaceutical Design 2010, 16:4225-4236 [PMID: 21184659] scopus 2-s2.0-79951716068 56. MA Gentile, S Melchiorre, C Emolo, M Moschioni, C Gianfaldoni, L Pancotto, I Ferlenghi, M Scarselli, W Pansegrau, D Veggi, M Merola, F Cantini, P Ruggiero, L Banci, V Masignani. Structural and functional characterization of the Streptococcus pneumoniae RrgB pilus backbone D1 domain. J. Biol. Chem. 2011 286:14588-14597 [PMID: 21367860] scopus 2-s2.0-79954588941 57. C Harfouche, S Filippini, C Gianfaldoni, P Ruggiero, M Moschioni, S Maccari, L Pancotto, L Arcidiacono, B Galletti, S Censini, E Mori, M Giuliani, C Facciotti, E Cartocci, S Savino, F Doro, M Pallaoro, S Nocadello, G Mancuso, M Haston, D Goldblatt, M Barocchi, M Pizza, R Rappuoli, V Masignani. RrgB321, a fusion protein of the three variants of the pneumococcal pilus backbone RrgB, is protective in vivo and elicits opsonic antibodies. Infect. Immun. 2012 80:451-460 [PMID:22083702] scopus 2-s2.0-84856579762 58. M Moschioni, G De Angelis, C Harfouche, E Bizzarri, S Filippini, E Mori, G Mancuso, F Doro, MA Barocchi, P Ruggiero, V Masignani. Immunization with the RrgB321 fusion protein protects mice against both High and Low pilus-expressing Streptococcus pneumoniae populations. Vaccine 2012, 30:1349-1356 [PMID:22210141] scopus 2-s2.0-84856566929 59. P Ruggiero. Helicobacter pylori infection: what’s new. Curr. Opin. Infect. Dis. 2012 35:337-344 [PMID: 22555448] scopus 2-s2.0-84861021045 60. A Pezzicoli, P Ruggiero, F Amerighi, JL Telford, M Soriani. Exogenous sialic acid transport contributes to GBS colonization of mouse mucosal surfaces. J. Infect. Dis. 2012 206:924-931 [PMID: 22829646] scopus 2-s2.0-84865779998 61. L Pancotto, G De Angelis, E Bizzarri, MA Barocchi, G Del Giudice, M Moschioni, P Ruggiero. Expression of the Streptococcus pneumoniae pilus-1 undergoes on and off switching during colonization in mice. Sci. Rep. 2013, 3:2040. doi:10.1038/srep02040 [PMID: 23784148] scopus 2-s2.0-84879665846 62. M Stein, P Ruggiero, R Rappuoli, F Bagnoli. Helicobacter pylori CagA: from pathogenic mechanisms to its use as an anti-cancer vaccine. Front. Immunol. 2013, 4:328. doi:10.3389/fimmu.2013.00328 [PMID: 24133496] 63. P Ruggiero, S Censini. Helicobacter pylori: a brief history of a still lacking vaccine. Diseases 2014, 2:187-208. doi: 10.3390/diseases2020187 64. P Ruggiero. Use of probiotics in the fight against Helicobacter pylori. World J Gastrointest Pathophysiol. 2014, 5:384-391. doi: 10.4291/wjgp.v5.i4.384 [PMID: 25400981] 65. F Bagnoli, MR Fontana, E Soldaini, R Mishra, L Fiaschi, E Cartocci, V Nardi-Dei, P Ruggiero, S Nosari, MG De Falco, G Lofano, S Marchi, B Galletti, P Mariotti, M Bacconi, A Torre, S Maccari, M Scarselli, CD Rinaudo, N Inoshima, S Savino, E Mori, S Rossi-Paccani, B Baudner, M Pallaoro, E Swennen, R Petracca, C Brettoni, S Liberatori, N Norais, E Monaci, JB Wardenburg, O Schneewind, DT O’Hagan, NM Valiante, G Bensi, S Bertholet, E De Gregorio, R Rappuoli, G Grandi. Vaccine composition formulated with a novel TLR7-dependent adjuvant induces high and broad protection against Staphylococcus aureus. PNAS 2015, 112:3680-3685 doi: 10.1073/pnas.1424924112 [PMID: 25775551] scopus 2-s2.0-84925422900 66. N Chiappini, R Cantisani, L Pancotto, P Ruggiero, D Rosa, A Manetti, A Romano, F Montagnani, S Bertholet, F Castellino, G Del Giudice. Innate Response Activator (IRA) B cells reside in human tonsils and internalize bacteria in vitro. PLoS One. 2015 10(6):e0129879. doi: 10.1371/journal.pone.0129879 [PMID: 26066485] scopus 2-s2.0-84936122447 67. J Mishra, P Ruggiero, F Bagnoli, R Rappuoli, M Stein. Helicobacter pylori: The Cancer Bug. In: Infection and Cancer: Bi-Directorial Interactions, MR Shurin et al. Editors, Springer International Publishing Switzerland, 2015, DOI 10.1007/978-3-319-20669-1_10 scopus 2-s2.0-84957651986 68. F Amerighi, M Valeri, D Donnarumma, S Maccari, M Moschioni, A Taddei, L Lapazio, W Pansegrau, S Buccato, G De Angelis, P Ruggiero, V Masignani, M Soriani, A Pezzicoli. Identification of a monoclonal antibody against pneumococcal pilus-1 ancillary protein impairing bacterial adhesion to human epithelial cells. J Infect Dis 2015, 213:516-522 [PMID: 26401026] scopus 2-s2.0-84960172602 69. D Casini, P Fontani, P Ruggiero, E Balducci, D Berti. A rapid ELISA method to improve the automated test throughput. J Anal & Bioanal Tech 2015, S13:005. doi:10.4172/2155-9872.S13-005 70. E Viciani, F Montagnani, S Tavarini, G Tordini, S Maccari, M Morandi, E Faenzi, C Biagini, A Romano, L Salerni, O Finco, S Lazzi, P Ruggiero, A De Luca, MA Barocchi, A Manetti. Paediatric obstructive sleep apnoea syndrome (OSAS) is associated with tonsil colonisation by Streptococcus pyogenes. Scientific Reports 2016, 6:20609. doi: 10.1038/srep20609 [PMID: 26860261] scopus 2-s2.0-84958069196 71. P Ruggiero. Helicobacter pylori CagA: the bacterial oncoprotein. In: Toxinology. Microbial Toxins. P Gopalakrishnakone, B Stiles, A Alape-Girón, JD Dubreuil, M Mandal Editors, Springer, 2016 [ISBN (online): 978-94-007-6725-6] DOI: 10.1007/978-94-007-6725-6_22-1 72. A Corrado, P Donato, S Maccari, R Cecchi, T Spadafina, L Arcidiacono, S Tavarini, C Sammicheli, D Laera, AG Manetti, P Ruggiero, B Galletti, S Nuti, E De Gregorio, S Bertholet, A Seubert, F Bagnoli, G Bensi G, E Chiarot. Staphylococcus aureus-dependent septic arthritis in murine knee joints: local immune response and beneficial effects of vaccination. Scientific Reports 2016, 6:38043. doi: 10.1038/srep38043 [PMID: 27901071] 73. E Viciani, F Montagnani, G Tordini, A Romano, L Salerni, A De Luca, P Ruggiero, AGO Manetti. Prevalence of M75 Streptococcus pyogenes strains harboring slaA gene in patients affected by pediatric obstructive sleep apnea syndrome in central Italy. Frontiers Microbiology, 2017, 8:294 [PMID: 28293224] doi: 10.3389/fmicb.2017.00294 74. E Talluri, L Pancotto, P Ruggiero, M Scarselli, E Balducci. CagL from Helicobacter pylori has ADP-ribosylation activity and exerts partial protective efficacy in mice. Arch Biochem Biophys, 2017, 635:102-109 [PMID: 29097311 doi: 10.1016/ 75. E Chiarot, A Spagnuolo, S Maccari, E Naimo, A Acquaviva, R Cecchi, B Galletti, M Fabbrini, E Mori, P Ruggiero, G Grandi, MR Fontana, G Bensi, I Margarit. Protective effect of Group B Streptococcus type-III polysaccharide conjugates against maternal colonization, ascending infection and neonatal transmission in rodent models. Sci Rep, 2018, 8:2593 [PMID: 29416049] doi: 10.1038/s41598-018-20609-5 76. P Malfertheiner, M Selgrad, T Wex, B Romi, E Borgogni, F Spensieri, L Zedda, P Ruggiero, L Pancotto, S Censini, E Palla, N Kanesa-Thasan, B Scharschmidt, R Rappuoli, DY Graham, F Schiavetti, G Del Giudice. Efficacy, immunogenicity, and safety of a parenteral vaccine against Helicobacter pylori in healthy volunteers challenged with a Cag-positive strain: a randomised, placebo-controlled phase 1/2 study. Lancet Gastroenterol Hepatol, 2018, 3:698-707 [PMID: 30042064] doi: 10.1016/S2468-1253(18)30125-0. PUBLICATIONS AND BOOKS FOR SCIENTIFIC DIVULGATION 1. Tagliabue A, P Ruggiero, D Boraschi: La famiglia interleuchina 1 e i suoi recettori. (The Interleukin-1 family and Its Receptors). Minerva Biotec 1992 4:232-235. [language: Italian] text and artwork 2. Tagliabue A, P Ruggiero, S Salvaneschi: Dal gene al farmaco. (From Gene to Drug) 1992. ISBN 88-85105-18-5, Libraria Editrice Internazionale, Milan, Italy. [language: Italian] text and artwork 3. Tagliabue A, D Boraschi, P Ruggiero, S Salvaneschi: I fattori di crescita e regolazione del sistema emopoietico e immunitario. (Growth and Regulation Factors of the Immune and Hematopoietic System) 1993. Editrice S.A.E. srl, Milan, Italy. [language: Italian] text and artwork 4. Tagliabue A, D Boraschi, P Ruggiero, S Salvaneschi: Terapie Biotecnologiche. (Biotechnological Therapies) 1996. Editrice S.A.E. srl, Milan, Italy. [language: Italian] text and artwork 5. Bertini R, C D'Ettorre, G Macchia, P Ruggiero, D Boraschi, A Tagliabue: Le chemiochine: un mondo a parte. (Chemokines: a world apart). Biotec 1996 1:54-64. [language: Italian] text and artwork TEACHING ACTIVITIES 1980-1982: Teacher of Sciences at High Schools (Liceo Classico Mariano Buracchi, Viterbo, Italy; Istituto Tecnico Industriale Statale Alessandro Volta, Viterbo, Italy; Liceo Scientifico Paolo Ruffini, Viterbo Italy); 2005-2013: lessons/seminars at the Department of Diagnostics, Clinical and Public Health Medicine, University of Modena and Reggio Emilia, Modena, Italy (June 10th, 2005; May 29th, 2007; January 25th, 2010; January 26th, 2012; January 14th, 2013); 2007-2011: yearly lessons at the Master “Metodologie farmaceutiche industriali”, University La Sapienza, Department of Chemistry and Pharmaceutical Technology, Roma, Italy (March 2, 2007; February 29, 2008; February 27, 2009; March 6, 2010; March 5th, 2011) ARTWORK Graphic art for meetings and courses of GCI (Gruppo di Cooperazione in Immunologia, currently Società Italiana di Immunologia, Immunologia Clinica e Allergologia) in 1988, 1989, 1996-1998, 2000 and 2002. Scientific illustrations or cartoons in the following scientific/divulgation publications: 1. D Boraschi et al: Interleuchine postmoderne. Biotec 1986 6:27-30 2. P Comoglio: Gli anticorpi anti-fosfotirosina per la diagnosi dei tumori. Biotec 1986 6:21-23 3. S Casalotti: Biologia cellulare dei neurorecettori. Biotec 1987 3:24-28 4. G De Libero, P Ricciardi-Castagnoli: I vettori retrovirali. Biotec 1987 3:35-37 5. V Pallini et al: L’immunoistochimica dalle origini al suo incontro con le biotecnologie. Biotec, Supplement 1987 6. Vaccini più sicuri con le biotecnologie and La produzione di interleuchina. In Experimenta ’89; Biotecnologie e ambiente (Ricerca e Innovazione 1983 3 suppl:28-30 ) 7. A Mantovani: Approcci molecolari in Immunologia. CLAS International, Brescia 1989 (ISBN 88091-29-5) (cover cartoon) 8. A Tagliabue: Reports: Vaccinazioni – duecento anni di ricerche. Meditime 1990 28:12-13 9. A Tagliabue: Reports: Vaccinazioni – le metodologie del futuro. Meditime 1990 29:12-13 10. A Tagliabue: Reports: Autoimmunità – le regole del gioco. Meditime 1991 5:12-13 11. A Tagliabue: Reports: Biotecnologie – La nuova strada della terapia. Meditime 1991 35:16-17 12. A Tagliabue:Alla ricerca del vaccino. Meditime 1991 27:2 13. A Tagliabue: La moda delle citochine. Meditime 1992 4:2 14. N Tannoia: La Talassemia – una realtà pugliese. Bari, 1994 (consultancy for illustrations) 15. E Porreca et al: Monocyte Chemotactic protein-1 (MCP-1): a possible target for therapeutic agents in atherosclerosis. Int. J. Immunopathol. Pharmacol. 1996 9:67-72 16. VM Fazio et al: Vaccini a DNA: l’immunizzazione genetica. Biotec 1996 5:24-25 17. Apoptosis cartoon, Trends in Biochemical Sciences 1997 22:109 18. International Society for Interferon and Cytokine Research, newsletter vol. 4, September 1997, p. 17 19. C D’Ettorre et al: Functional epitope mapping of human interleukin-1β by surface plasmon resonance. European Cytokine Network 1997 8:161-171 20. International Society for Interferon and Cytokine Research, newsletter vol. 4, December 1997, p. 7 21. Trasfezione e vettori retrovirali. Giornale del Medico 12 Ottobre 1998, p.28 22. A Cossarizza, D Boraschi: Apoptosis – A Laboratory Manual of Experimental Methods, Graphic Press 1998 (cover cartoon) 23. MP Abbracchio et al: Disclosing apoptosis in the CNS. Trends in Pharmacological Sciences 1999 20:129-131 PATENTS (including references according to EPO – Espacenet) 1. P Ruggiero, R Petracca: Method for the immunolocalization of antigens with the use of antibodies directed against epitopes of non-glucidic nature. Eur. Pat. Appl. 88201812.0 August 25, 1988; IT Appl. 2179287, September 4, 1987. US4954433 (A) 1990-09-04 - Application number: US19880239642 19880902 - Priority number: IT19870021792 19870904 - Also published as: EP0306082 (A2) EP0306082 (A3) IT1230673 (B) 2. L Nencioni, M Bugnoli, R Manetti, I Marsili, P Ruggiero, R Rappuoli: Acellular anti-pertussis vaccines. Eur. Pat. Appl. 91109883.8 June17, 1991; IT Appl. 2071090, June 21, 1990. APPLICATION EP19910109883, PUBLICATION EP0462534 A2. IT1248735 (B) 1995-01-26 - Application number: IT19900020710 19900621 - Priority number: IT19900020710 19900621 - Also published as: IT9020710 (A1) EP0462534 (A2) EP0462534 (A3) JPH04368337 (A) JP2690215 (B2) CA2045071 (A1) 3. D Boraschi, P Bossù, P Ruggiero, G Macchia, A Tagliabue, F Frigerio, R Grifantini, G Frascotti, G Grandi: Antagonisti recettoriali di IL-1 ad aumentata attività inibitoria. (IL-1 receptor antagonists with enhanced inhibitory activity). IT Appl. September 21, 1994, n. MI94/A 001916. APPLICATION US 08/809,185, PUBLICATION US5922573 A. US5922573 (A) 1999-07-13 - Application number: US19970809185 19970415 - Priority numbers: WO1995EP03708 19950920 ; IT1994MI01916 19940921 - Also published as: WO9609323 (A1) ITMI941916 (A1) IT1269989 (B) EP0782584 (A1) 4. G Maurizi, P Bossù, D Boraschi, P Ruggiero, A Tagliabue: Metodo di recupero di prodotti biologicamente attivi da microrganismi. (Method for the recovery of biologically active products from microrganisms). IT Appl. November 15, 1994, n. MI94/A 002308. IT1270697 (B) 1997-05-07 - Application number: IT1994MI02308 19941115 - Priority number: IT1994MI02308 19941115 - Also published as: ITMI942308 (A1) 5. D Boraschi, P Bossù, P Ruggiero, G Macchia, U Visconti, G Frascotti, F Frigerio, R Grifantini, G Grandi, A Tagliabue: Mutanti di IL-1beta con capacità selettiva di inibizione di IL-1RII. (IL-1beta mutants with selective IL-1RII inhibitory ability). IT Appl. March 31, 1995, n. MI95/A 000660. ITMI970475 (A1) 1998-09-04 - Application number: IT1997MI00475 19970304 - Priority number: IT1997MI00475 19970304 - Also published as: IT1290019 (B1); ITMI960577 (A1) ― 1997-09-23 - Application number: IT1996MI00577 19960322 - Priority number: IT1996MI00577 19960322; ITMI950660 (A1) 1996-03-22 - Application number: IT1995MI00660 19950331 - Priority number: IT1995MI00660 19950331 6. A Tagliabue, D Boraschi, P Bossù, G Macchia, G Maurizi, S Porzio, P Ruggiero: Microorganisms as therapeutic delivery systems. PCT/EP n. 95/03921, October 4, 1995. U.S.A. Appl. U.S.P. 08/539,368 October 5, 1995. APPLICATION PCT/EP1995/003921, PUBLICATION WO1996011277 A1. EP0784689 (A1) 1997-07-23 - Application number: EP19950935431 19951004 - Priority number(s): WO1995EP03921 19951004 ; IT1994MI02025 19941005 - Also published as: WO9611277 (A1) JPH10506791 (A) ITMI942025 (A1) IT1270123 (B) CA2201721 (A1) AU3745395 (A) 7. P Ruggiero, G Maurizi, V Di Cioccio, A Ciabini: Molecular weight markers for western blotting. PCT/EP01/02890, March 15, 2001. Publication number: 20050064537. US2005064537 (A1) 2005-03-24 - Application number: US20040221854 20040517 - Priority numbers: WO2001EP02890 20010315 ; IT2000MI00552 20000317 - Also published as: WO0168899 (A2) WO0168899 (A3) JP2003526799 (A) ITMI20000552 (A1) IT1318400 (B1) EP1264184 (A2) EP1264184 (B1) CA2399112 (A1) AU5220201 (A) AT315232 (T) 8. C Donati, A Muzzi, V Masignani, F Bagnoli, P Ruggiero, M Barocchi: Compositions comprising pneumococcal antigens, WO/2009/016515, PCT/IB2008/002866, filing date 01.08.2008, publication date 05.02.2009. Publication number: 20110243976. PL2572726 (T3) 2016-12-30 - Application number: PL20120197549T 20080801 - Priority numbers: GB20070014963 20070801 ; US20070966866P 20070829 - Also published as: WO2009016515 (A2) WO2009016515 (A3) US2014348868 (A1) US2011243976 (A1) SI2572726 (T1) JP2014034578 (A) JP5838193 (B2) JP2010535025 (A) HRP20160685 (T1) ES2580957 (T3) EP2572726 (A1) EP2572726 (B1) EP2197484 (A2) EA201070200 (A1) EA018137 (B1) DK2572726 (T3) CO6260103 (A2) CN104001165 (A) CN101801409 (A) CA2695169 (A1) AU2008281438 (A1) 9. V Masignani, M Barocchi, M Moschioni, P Ruggiero: Combinations of pneumococcal RrgB clades, WO/2010/140119, PCT/IB2010/052445, filing date 01.06.2010, publication date 09.12.2010. Publication number: 20120076814. ZA201109046 (B) 2013-05-29 - Application number: ZA20110009046 20111208 - Priority numbers: US20090217629P 20090601 ; US20090254426P 20091023 ; US20100314203P 20100316 ; US20100318926P 20100330 ; WO2010IB52445 20100601 - Also published as: WO2010140119 (A1) US2012076814 (A1) US2011020385 (A1) US8609106 (B2) SG176285 (A1) RU2011154363 (A) MX2011012854 (A) KR20120035168 (A) JP2015227376 (A) JP2012528848 (A) JP6008741 (B2) ES2531115 (T3) EP2437777 (A1) EP2437777 (B1) CO6470864 (A2) CN102481354 (A) CA2764022 (A1) BRPI1011857 (A2) AU2010255356 (A1) 10. M Giuliani and P Ruggiero: Combination of meningococcal factor H and pneumococcal saccharide conjugates WO/2010/109324, PCT/IB2010/000734, filing date 24.03.2010, publication date 30.09.2010. Publication number: 20120064103. BRPI1009829 (A2) 2016-11-16 - Application number: BR2010PI09829 20100324 - Priority numbers: WO2010IB00734 20100324 ; US20090163005P 20090324 ; US20090270407P 20090707 - Also published as: WO2010109324 (A1) WO2010109324 (A9) US2012064103 (A1) RU2011142774 (A) RU2555757 (C2) NZ595291 (A) JP2015028081 (A) JP2012521403 (A) JP5668049 (B2) EP3017826 (A1) EP2411045 (A1) CN102438649 (A) CL2015002157 (A1) CA2756533 (A1) AU2010227220 (A1) AU2010227220 (B2); AU2014268243 (A1) 2015-01-15 - Application number: AU20140268243 20141128 - Priority numbers: AU20100227220 20100324 ; AU20140268243 20141128 - Also published as: AU2014268243 (B2) MEMBERSHIPS  1996-1999: International Society for Interferon & Cytokine Research (ISICR)  1998-2008: Società Italiana di Immunologia, Immunologia Clinica e Allergologia (SIICA, previously GCI) business memberships:  2000-2006: Chiron srl: Internal Management Committee (IMC)  2006-2012: Novartis Vaccines & Diagnostics srl: Vaccine Research Leadership Forum (VRLF)  2013-2015: Novartis Vaccines & Diagnostics srl: Leadership Team Siena - 1 (LTS-1)  2014-2015: Novartis Vaccines & Diagnostics srl: Staphylococcus aureus Project Core Team REVIEWING ACTIVITY Ad hoc reviewer for the following journals: Biomedical Journals British Microbiology Research Journal DNA and Cell Biology European Archives of Oto-Rhino-Laryngology and Head & Neck European Journal of Medicinal Plants Expert Review of Vaccines Frontiers in Cellular and Infection Microbiology Future Microbiology Health Care: Current Reviews Helicobacter International Journal of Tropical Disease & Health Journal of Applied Microbiology Journal of Chemotherapy Journal of Pediatric Biochemistry Journal of Translational Medicine Nature Communications PLOSone Scandinavian Journal of Infectious Diseases Vaccine