Course
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Credits
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Scientific Disciplinary Sector Code
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Contact Hours
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Exercise Hours
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Laboratory Hours
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Personal Study Hours
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Type of Activity
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Optional materials and exam in a foreign language
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Language
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18374 -
Miglioramento genetico e biotecnologie del seme
(objectives)
1) Knowledge and understanding: the course aim to provide the student with the basic principles of crop breeding, variety registration and seed production. 2) Applied knowledge and understanding: theoretical and practical aspects of reproductive biology of higher plants are addressed including those modifications of the reproductive system that are of practical interest. Additional teaching objectives are the collection, storage and evaluation of genetic resources, the development of plant breeding schemes, the characterization, reproduction, conservative selection of plant varieties, the adoption of advanced strategies for the control of reproductive biology and the co-existence of conventional and genetically modified crops. 3) Making judgements: the advantages and disadvantages of each presented technology are illustrated, to develop the student's critical sense. 4) Communication skills: students are asked questions during the classes, both to keep their attention and to teach them to ask questions and give adequate answers. 5) Learning skills: the theoretical bases of processes underlying plant reproductive biology, plant breeding schemes and stragetgies to select improved varieties through conventional breeding.
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MAZZUCATO Andrea
( syllabus)
- Introduction, history and aim of plant breeding, concept of ideotype. - Biology of plant reproduction: floral morphology, macro and microsporogenesis, macro and microgametogenesis, pollination, progamic phase, fertilization, embryogenesis, seed and fruit development. Molecular biology of flowering induction and flower development, genes controlling inflorescence, flower and floral organs identity, ABC(DE) model. Mode of reproduction (sexual reproduction, vegetative propagation, apomixis), sex determination (hermaphroditism, monoecism, dioecism), mating system (autogamy and allogamy), experimental characterization of plant reproductive system and of outcrossing rate. Male sterility (genetic, cytoplasmic and genetic-cytoplasmic, functional and conditional). Self-incompatibility (sporophytic and gametophytic). Apomixis, cytoembryological bases, genetic control, breeding of obligate and facultative apomictic species, perspectives of transferring apomixis to sexual species. Fruit set and development, parthenocarpy, biotechnological approaches to control fruit set. Fruit ripening, mutants affected in ripening and in pigment accumulation. - Sources of genetic variability: gene pool concept, germplasm collection and storage, seed banks, germplasm evaluation, variability induced by mutagenesis, somaclonal variation. - Intra and interspecific cross, sexual interspecific barriers, biotechnologies to facilitate interspecific crossing, in vitro fertilization and embryo rescue. - Theory of selection: principles of theory of selection for monogenic and polygenic characters, penetrance and expressivity. - Genetic population structure in autogamous, allogamous, vegetatively propagated and apomictic species. - Breeding schemes for autogamous species: selection in existing populations: mass selection and pure line selection. Selection in segregating populations: pedigree, bulk and single seed descent methods, double haploids. Backcross method to transfer a dominant and a recessive allele, linkage drag, principles of marker assisted selection, multiline varieties, F1 hybrids in autogamous species. - Breeding schemes for allogamous species: mass selection, recurrent selection, synthetic varieties, F1 hybrids in allogamous species, use of male sterility in hybrid production schemes. - Principles of genetics of seed production: evaluation of new varieties, subscription to the Register of Varieties, maintenance selection and seed production, isolation, generations of seed multiplication. Legislation related to seed production and marketing. Biotechnologies for the protection of plant varieties (molecular markers for distinctiveness), estimation of genic flux and gene flux containment in conventional and genetically modified varieties.
Seminars: students will be invited to attend one or two seminars focussing on technical and/or scientific aspects related to topics of the course. Practical activities: practical activity will be dedicated to the knowledge of genetic variability in a crop species, to the examination of mutants involved in reproductive biology, to technicalities to carry out controlled crosses and to a visit to a company or experimental station involved in breeding and/or seed production of crop species.
( reference books)
Barcaccia G. e Falcinelli M., Genetica e genomica, vol. II, 2005, Miglioramento genetico, Liguori. Lorenzetti F. et al., Miglioramento genetico delle piante agrarie, 2018, Edagricole. Ciriciofolo E. e Benincasa, Sementi: Biologia, produzione e tecnologia, 2018 Edagricole. Materiale fornito dal docente tramite il Portale Docente.
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6
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AGR/07
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48
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Core compulsory activities
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ITA |
18376 -
Chimica delle sostanze organiche naturali
(objectives)
The course introduces students to the world of natural products present in the plant world (secondary metabolites), correlating them with biogenetic precursors. For each family of products, the structural aspects; the biological activities; the applications in the agronomic, food, cosmetic, pharmaceutical and nutraceutical fields. The techniques of extraction, purification and structural characterization of the natural products are also described. The expected results are the following: 1) Knowledge and understanding. Provide knowledge of natural substances, particularly about the chemical structure and main biological activities. 2) Applying knowledge and understanding. the potential uses of natural substances in the food, nutraceutical, agronomic, cosmetic, and pharmaceutical fields. 3) Making judgements. Develop the ability to construe the experimental results. 4) Communication skills. Develop the ability to describe to describe course topics with language properties, clarity, synthesis skills and critical meaning. 5) Learning skills. Learn the tools to study of natural substances.
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BERNINI Roberta
( syllabus)
The course is divided into two parts. The first, of 32 hours, includes lectures; the second, lasting 16 hours, includes exercises, seminars, laboratory activities. Introduction to the course. Description of the program, of the training objectives and of the expected results. Description of the examination method and evaluation criteria. Presentation of the international Scopus and Web Science databases. Chemical structure, biological properties and applications in the agronomic, food, cosmetic, pharmaceutical and nutraceutical fields of the main natural organic substances (secondary metabolites) present in the plant world described by following the biogenetic pathways. Metabolites derived from the acetate pathway. Saturated fatty acids. Unsaturated fatty acids. Uncommon (acetylenic and branched-chain) fatty acids. Prostaglandins. Thromboxanes. Leukotrienes. Macrolides. Statins. Simple phenols. Tetracyclines. Anthraquinones. Antrones. Diantrones. Anarcardic acids. Urusciols. Aflatoxins. Cannabinoids. Metabolites derived from the shikimate pathway. Aromatic amino acids. Benzoic acids. Cinnamic acids. Lignans. Lignin. Phenylpropanes. Coumarins. Stilbenes. Flavonoids. Flavonolignans. Isoflavonoids. Benzoquinones. Naphthoquinones. Anthraquinones. Terpenoid quinones. Hydrolyzable tannins. Condensed tannins. Metabolites derived from the mevalonate pathway. Hemiterpenes. Linear, cyclic and irregular monoterpenes. Iridoids. Secoiridoids. Sesquiterpenes. Diterpenes. Sesterterpenes. Triterpenes. Tetraterpenes. Higher terpenoids. Steroids. Alkaloids derived from ornithine, lysine, nicotinic acid, tyrosine, tryptophan, anthranilic acid, histidine, amination reactions; purine alkaloids. Free radicals. Oxidative stress. Antioxidants. In vitro methods for determining antioxidant activity. Traditional and innovative extraction techniques of natural substances. Techniques of separation and characterization of natural substances. Chromatographic techniques. Nuclear Magnetic Resonance.
( reference books)
For a good exam preparation, the following textbook is recommended: “Medicinal Natural Product. A Biosynthetic Approach” - Paul M. Dewick. John Wiley Sons, Ltd. On the Moodle platform, on the course page, the slides of all the lessons held by the teacher are available.
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6
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CHIM/06
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48
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Core compulsory activities
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ITA |
18377 -
Biotecnologie vegetali e prodotti farmaceutici
(objectives)
The course is aimed to promote in depth cultural, theoretical and experimental knowledge on the design, production and application of recombinant molecules with a high degree of added value in the pharmaceutical field. Several traditional biological systems for the production of biopharmaceuticals are taken into account. Also of particular importance it is given to plants as innovative and sustainable bio-factory both economically and environmentally.
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SANTI Luca
( syllabus)
The course is mainly divided into three sections: an initial more general section, a specific section focused on the use of plants for the production of recombinant proteins and a third section where students present in the classroom, in Italian, a scientific paper written in English. Moreover during the course three seminars, taught by guest experts, are planned on specific topics. In the initial section general aims of pharmaceutical biotechnology and pharmaceuticals obtained through biotechnological production are introduced, in this regard some definitions of Biopharmaceutical are provided together with specific examples and the general picture of their worldwide market. In addition, biotech drugs are compared to traditional drugs with particular reference to new approaches for their realization. In this context also recapitulative concepts on recombinant DNA technology strictly applied to pharmaceuticals are provided. All main biological systems for production are analysed: bacteria especially E. coli, yeasts, insect cells, different types of mammalian cells and chicken eggs. Production issues for large scale issues at both upstream and downstream processing level are also addressed in the light of international quality standards and of "Good Manufacturing Practices" (GMP) procedures. In particular specific lessons are dedicated to biofermentations, product processing, techniques of analysis and purification of biological macromolecules and their formulation for their release on the market. After an introduction to immunity, immunogenicity and the immune system of humans, all the main types of biopharmaceuticals: vaccines, polyclonal antibodies, monoclonal antibodies, immunomodulatory proteins and enzymes, in the context of the pathologies to which these drugs are directed to, are defined and described also using specific case studies. At the conclusion of the general section recent developments of nanotechnology in biomedicine and diagnostics and possible future applications in the biopharmaceutical industry are discussed. The second section, all centered on the use of plants for the expression of recombinant polypeptides with pharmaceutical activity, starts with the in depth discussion of plant viruses from a molecular point of view for the importance they have had and continue to have in the development of expression systems. In particular the various strategies of expression and replication of the best known plant viruses are addressed and the systems that make use of these viruses for the heterologous transient expression in plants analyzed in detail. Subsequently the most used plants in biopharmaceutical development (tobacco, benthamiana, rice, maize, potato and tomato) and the different types of genetic modification applicable to these species are addressed (stable nuclear transformation, plastid transformation and transient transformation). Numerous case studies concerning plants developed biopharmaceuticals will be presented and analyzed in detail to illustrate the different approaches in relation to the type of biopharmaceutical, the pathology and the receiving species (for human or veterinary use). The third section focuses on the acquisition of skills in the analysis of scientific international manuscripts and on the acquisition of skills in the presentation and exposure of technical content. To this end, students choose an article from a list of publications selected by the teacher and prepare a power point presentation of about 20 minutes to explain to their class mates, the general theme, the experimental approach, the results and prospects of the work. All selected publications concern the design, implementation and development of biopharmaceuticals in plants. The three seminars that take place during the course are: i) elements of immunology held by Dr. Selene Baschieri from the ENEA Casaccia research center; ii) principles of vaccinology held by Dr. Chiara Lico from ENEA Casaccia research center.
( reference books)
Biotecnologie farmaceutiche; Crommelin D. J., Sindelar R. D.; Zanichelli; 2000; ISBN: 8808094197. Elementi di Virologia Vegetale; Giunchedi, Conti, Gallitelli, Martelli; 2007; ISBN: 978-88-299-1838-6.
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6
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BIO/15
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48
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Related or supplementary learning activities
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ITA |
18373 -
Biotecnologie e nutraceutica delle produzioni animali
(objectives)
The educational objectives of the course are to provide the student with knowledge on the principles underlying the improvement of animals in livestock production, the biotechnologies of animal reproduction in livestock production, molecular biology applied to animal production, study of the nutraceutical aspects of animal products and nutraceutical evaluation of biolipids and natural molecules for the improvement of animal health and welfare. We will therefore address issues concerning animal experimentation and possible alternative techniques, basic theoretical knowledge on in vitro cultures of animal cells and tissues and their applications, methodologies related to the production of transgenic animals and the technologies most used in the quality and traceability of products of animal origin, in the control of genetic improvement and in the maintenance of animal biodiversity. Methods such as nucleic acid extraction, Polymerase Chain Reaction, sanger and new generation sequencing, microarrays, will be addressed to analyze in detail topics such as the use of markers useful for diagnostic and selective purposes, gene expression analysis, Research of polymorphisms associated with genetic improvement using molecular genetic techniques, traceability of products of animal origin.
Expected learning outcomes: At the end of the course the students will be able to: - acquire knowledge on the systems of genetic improvement in animal production and on the application of genomics to the processes of genetic improvement; - know the application of biotechnology to animal breeding; - acquire knowledge and skill in the use of cell cultures for physiology studies in the animal field.
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BASIRICO' Loredana
( syllabus)
1. Production systems 2. Qualitative aspects of animal production 3. Biotechnologies of reproduction 4. Genetic improvement to. Quantitative genetics b. Genomics 5. Nutraceuticals of products of animal origin 6. GMOs in animal production 7. On-farm exercises: visit to agro-zootechnical farms 8. Laboratory exercises: cell cultures, and…. 9. Seminars
( reference books)
Power point collection: Moodle and DropBox Recommended text: will be indicated when needed
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6
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AGR/18
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48
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Core compulsory activities
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ITA |