CHIMICA BIOLOGICA
(objectives)
The course aims to provide a general framework for understanding the biochemical bases of cell biology and to provide the basis for further studies in Biochemical Methods and Food Science. The course will introduce students to the knowledge of the structure and function of the main biological molecules: proteins, carbohydrates and lipids. In addition, general energy concepts will be provided: state functions (enthalpy, entropy and free energy), standard status - high energy compounds as well as knowledge of cellular metabolism and its regulation in relation to physiological needs in humans. Experimental laboratory is also provided allowing preparation of different solutions, diluitions and buffers.
EXPECTED LEARNING RESULTS
Knowledge and
understanding: During the course, the knowledge of the structural and functional characteristics of biomolecules, enzyme properties, genetic material organization and the main metabolic processes of carbohydrates, lipids and proteins will be achieved.
Applying knowledge
and understanding: In addition to the knowledge gained through the study of Biological Chemistry, students will be encouraged to deepen the application potential of this course in the field of biological, biochemical and biomedical analysis.
Making judgements: The course provides links to other related disciplines to provide a broad but in-depth view of the cellular and molecular bases of cell behavior that will allow students to critically address a wide range of biomedical issues with autonomy of judgment. Moreover, during the course students will be able to develop logical and deductive arguments useful to work autonomously and for inclusion in the world of work.
Communication skills: During the lessons students will be stimulated to discuss and compare different point of views in order to develop their communicative abilities that will be verified during preliminary examination (written test) and final oral examinations at the end of training activities.
Learning skills: Students should be able to describe the scientific topics related to Biological Chemistry in written and oral form. This skill will be developed through the active involvement of students through oral class discussions and practical experiences during the hours dedicated to the experimental laboratory.
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Code
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15288 |
Language
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ITA |
Type of certificate
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Profit certificate
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Credits
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9
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Scientific Disciplinary Sector Code
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BIO/10
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Contact Hours
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64
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Exercise Hours
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8
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Type of Activity
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Basic compulsory activities
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Teacher
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PROIETTI Silvia
(syllabus)
First part of the program (4 CFU) General aspects of Biochemistry Weak interactions in aqueous systems. Chemical-physical properties of water. Ionization of water, weak acids and basics. Buffers.
Composition and structure of proteins Amino acids e their general properties. Peptide bond. Levels of organization of proteins: primary, secondary, tertiary and quaternary structures. Functional aspects of proteins. Myoglobin and haemoglobin.
Enzymes Fundamental concepts of enzymatic activity. Enzyme nomenclature, specificity, coenzymes and cofactors. Activation energy end role of enzymes. Overview of enzymatic kinetics.
Lipids, biological membranes and membrane proteins Fatty acids. Triacylglycerols. Glycerophospholipids. Lipidic and proteic components of biological membranes. Composition and architecture of biological membranes. Membrane proteins.
Carbohydrates Structure and function of carbohydrates. Oligosaccharides. Polysaccharides.
(reference books)
Nelson DL, Cox MM. Introduzione alla Biochimica di Lenhninger. 5a Edizione (2015) Zanichelli, Bologna. Voet D, Voet JG, Pratt CW. Fondamenti di Biochimica 3a Edizione (2013) Zanichelli, Bologna. Nelson DL, Cox MM. I principi di Biochimica di Lenhninger. 6a Edizione (2014) Zanichelli, Bologna. Berg JM, Tymoczko JL, Stryer L. Biochimica 7a Edizione (2012) Zanichelli, Bologna.
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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not mandatory
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Evaluation methods
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Oral exam
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Teacher
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CARUSO Carla
(syllabus)
Second part of the program (4 CFU)
Introduction to Metabolism Catabolism and Anabolism. Experimental approach to the study of Metabolism. Thermodynamics of phosphorus-containing compounds: ATP, and compounds with high energy. Electron transporters. Oxide-reduction reactions.
Carbohydrate Metabolism Glycolysis. Lactic fermentation. Reduction equivalent transport from cytoplasm to mitochondria. Krebs cycle. Pentose Shunt. Glycogen metabolism. Carbohydrates metabolism regulation.
Bioenergetics Compound with high energy. Electron transport. Oxidative phosphorylation. Oxidative metabolism control.
Lipid Metabolism Digestion, absorption and lipid transport. Fatty acids oxidation. Ketone bodies. Fatty acids biosynthesis. Regulation of fatty acids metabolism.
Protein Metabolism Fate of amino acid carbon skeleton. Deamination of amino acids: transamination and oxidative deamination in catabolic process. Reductive amination and transamination in anabolic process. Mode of action of glutamine synthetase and glutaminase. Urea cycle. Crosstalk between Krebs and urea cycles and gluconeogenesis. Gluconeogenetic and ketogenetic amino acids.
(reference books)
Nelson DL, Cox MM. Introduzione alla Biochimica di Lenhninger. 5a Edizione (2015) Zanichelli, Bologna. Voet D, Voet JG, Pratt CW. Fondamenti di Biochimica 3a Edizione (2013) Zanichelli, Bologna. Nelson DL, Cox MM. I principi di Biochimica di Lenhninger. 6a Edizione (2014) Zanichelli, Bologna. Berg JM, Tymoczko JL, Stryer L. Biochimica 7a Edizione (2012) Zanichelli, Bologna.
Non-attending students are encouraged to contact the teacher for information on the program, teaching materials and how to assess their achievement.
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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not mandatory
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Evaluation methods
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Oral exam
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