Teacher
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GEVI Federica
(syllabus)
Exposure to environmental contaminants. Toxicity of environmental contaminants towards cellular components, signs of genotoxicity. Genes and tumors, heredity of cancer risk. Molecular aspects of tumorigenesis. Cell death, apoptosis and necrosis in response to genotoxic agents. Pollutants in the environment: origin, diffusion, accumulation. Classification of environmental toxic substances. Trace elements, heavy metals, metals bound to organic compounds. Inorganic pollutants, asbestos, alkalinity, salinity, acidity. Trace organic pollutants (PCBs, PBDEs, dioxins, furans, PAHs, pesticides). Molecular methods for the study of the environment. PCR, analysis by agarose gel electrophoresis, cloning, sequencing. Real-time PCR. Application of molecular techniques to the study and isolation of environmental DNA, genetic fingerprinting techniques ARDRA, SSCP, T-RFLP, DGGE, RISA, LH-PCR, RAPD. DNA / RNA Stable isotope probing. Fluorescent in situ hybridization (FISH, CARD-FISH, Raman-FISH, Nano-SIMS). Microarray. Analytical methodologies for the determination of all classes of environmental pollutants (analytical spectroscopies, electroanalytical techniques, environmental techniques, gas chromatography, mass spectrometry for quantitative and qualitative analysis of halogenated compounds: (PCB, PCDD, PCDF and PBDE .) Liquid chromatography, mass spectrometry (LC / MS) for the qualitative and quantitative analysis of polar and thermolabile environmental pollutants, inductively coupled plasma mass spectrometry (ICP / MS) in environmental analysis.
(reference books)
Molecular biology. Principles and techniques. Michael M. Cox, Jennifer Doudna, Michael O'Donnell. Zanichelli 2013. Power point and educational material provided by the teacher during the lessons. The teacher will communicate at the beginning of the course any links where you can download the educational material.
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