Teacher
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MULEO Rosario
(syllabus)
Plant propagation and nursery industries. Plant organs and tissues. Certification of plant materials, patents and novelties. Organization of a micropropagation lab, culture media, components, physiological and morphogenic response of explants, choice of plant material. Multiplication and de novo shoot organogenesis. Cell culture and their application. In vitro culture and germplasm preservation. Embryo rescue, Embryo culture and techniques to overcome incompatibility (mentor pollen, in vitro pollination ecc…). In vitro culture for breeding and genetics (somaclonal variation, protoplast fusion, hybrids, molecules for selective pressure, elicitors). Genetic engineering: a) Agrobacterium spp., b) PEG, c) electroporation d) biolostic, e) analysis of scientific paper
(reference books)
-material provided by the teacher - Pierik R. L. 1999, In Vitro Culture of Higher Plants , Kluwer Academic Publishers - Triggiano et al 2010, English version - Hartman e Kester Propagazione delle piante. EDAGRICOLE
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