Teacher
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TIEZZI Antonio
(syllabus)
Program of the Course of Biotechnology of Plant Molecules What a plant is and what it is made of: a brief summary of the root, the stem, the leaf and the reproductive system. Plant molecules have accompanied humanity's journey. What is a molecule. The cell is a set of molecules. The cell is a system in balance, internal balance and in balance with the outside. Pathology as a result of impaired balance. Primary metabolites, the molecules of all cells, and their biosynthesis: nucleic acids, proteins, lipids, polysaccharides, vitamins. Biological activities of primary metabolites. Methodologies for the extraction and purification of primary metabolites. Secondary metabolites. Classification of secondary metabolites and their biosynthesis: terpenes and terpenoids, phenolic compounds, alkaloids. Biological activities of secondary metabolites. Methodologies for the extraction and purification of secondary metabolites. The biological test on bacterial, plant and animal cell models. What is the biological model; choice of the biological model. Brief introduction to the identification and culture conditions of the biological model. Cytoplasmic structures possible targets of secondary metabolites. Examples of human applications of molecules of plant origin in our daily lives. Examples of applications of vegetable molecules for environmental, animal and human health use. Into the laboratory. The search for new molecules. What is a research laboratory. Behavioral principles within a laboratory. The concept of the research team. The multidisciplinary approach to research. The research procedure in the field of plant molecules: the alternation and synergy of the chemical investigation and the biological investigation. The choice of plants to investigate; past knowledge and current knowledge, bibliographic research. The preparation of samples. Chemical investigations: chromatography methods. Introduction to mass spectrometry and nuclear magnetic resonance. Biological investigations: identification and culture of the biological model and sample administration; evaluation of the biological effect. Investigations bu the Cytofluorimeter. What a scientific publication is and how it is structured. Writing a work based on the results obtained from laboratory experience.
(reference books)
The slides showed during the Course and articles and handouts will be provided to the students.
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