PRINCIPLES OF SCIENCES "OMIC"
Introduction:
• "omics" sciences and biology of complex systems.
• The hypothesis revolution in retrospect and the search for new hypotheses.
• What are the "omic" sciences ?.
• From proteins to proteomics, metabolomics to the metabolites and lipids to Lipidomics.
• Relationship of proteomics and other post-genomic science.
technological platforms:
• Preparation of the sample (plants, bacteria, animals): protein extract preparation for DIGE analysis, general guidelines for sample preparation for LC-MS analysis.
• protein expression analysis, metabolites and lipids by electrophoretic techniques
or Applications of 'SDS-PAGE and dell'IEF proteomics, metabolomics and lipidomics.
or Differential Gel Electrophoresis (DIGE), 2D-DIGE saturation
or capillary electrophoresis (CE), two-dimensional electrophoresis (2DE).
or Applications of 2-DE in proteomics: limits and perspectives.
or Blue Native electrophoresis on polyacrylamide gel (Blue Native PAGE).
or Clear Native PAGE (CN-PAGE).
Electrophoresis or 'gel free'
or Electrophoresis on cellulose acetate
• Methods for post analysis disclosure: reverse coloring, fluorescent dyes, Image Analysis for multiplexing technologies
• Methods of detection of post-translational modifications in mass spectrometry:
or glycosylation, phosphorylation, nitration, carbonylation
• protein expression analysis using chromatographic techniques:
or proteomic approach Bottom-up
or proteomic approach Middle-down
Approach or top-down proteomics
or Multidimensional Separations
• Mass Spectrometry: introduction and concepts of resolution and accuracy
o The most used sources in the field of proteomics: MALDI, ESI
o The most used sources in Metabolomics: EI
o The most used sources in the field lipidomic: ESI, MALDI
or analyzers: quadrupole, TOF and ion trap, Orbitrap, hybrid
• The tandem mass spectrometry (MS \ MS)
quantitative methods: liquid chromatography system (LC), reverse phase (RP) coupled to mass spectrometry analysis.
or strategies relative quantification based on labeling with stable isotopes (iTRAQ, SILAC spike-in, Super-SILAC and SILAC organisms, ICAT, O18)
or relative quantification strategies without marking ( "label-free" methods: intensity-based and spectral-counting, calculation of the PAI and dell'emPAI).
or proteomics, metabolomics and targeted quantitative Lipidomics: SRM
hyphenated techniques: GC-MS, CE-MS, LC-MS \ MS, MudPIT

Anna Maria Timperio

There is not a specific text, the teacher will take care to report appropriate articles and magazines during the year.