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Teacher
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FICCA Anna Grazia
(syllabus)
Program
Historical aspects : from the discovery of DNA to the genetic code and structure of nucleic acids.
a) Structure of DNA and RNA: nucleotides, conformations of helices A, B , C , denaturation and renaturation of the double helix.
b) Organization of DNA supercoiling , the histone proteins and the levels of organization of chromatin : nucleosome . Analysis of chromatin by enzymatic digestion with nuclease .
c) DNA replication : replicon model , identification of the origin of replication in prokaryotes and eukaryotes : structural and functional characteristics of the proteins involved in the process of replication, Okazaki fragments , the primers , the DNA polymerase I -II-III .
Transcription .
a) The Control of Transcription in prokaryotes . Structure of ”Operon” . Operator Sites and Promoters ( strong and weak promoters ). RNA polymerase and sigma factors .
b ) Repressors and activators : the Lac operon and the operon araBAD
c) The attenuation : the Trp operon .
d) The RNA polymerase in eukaryotes : structure, promoters, initiation, elongation and termination.
e) The eukaryotic mRNA : capping, the tails of poly ( A), mRNA splicing .
Translation
a) The genetic code
b) Transfer RNA : structure, aminoacyl-tRNA synthetases , proofreading and correction, the pairing oscillating , the suppressor mutation no sense .
c) Ribosomes : composition, structure , antibiotic inhibitors of protein synthesis.
d ) Beginning the translation: GTP , the Shine- Dalgarno sequence , the initiation codon , the protein factors
e) The elongation : the binding of tRNA , the transpeptidation , translocation , protein elongation factors .
f) Termination
The study of genes : cloning techniques ; the cloning vectors , gene amplification (PCR ) ; DNA sequencing ; analysis of the promoters ( use of reporter genes )
(reference books)
Amaldi F., Benedetti P. Pesole G., Plevani P., "Biologia molecolare" terza ed. (2018) ed. Casa editrice Ambrosiana
Zlatanova J., van Holde K.E. Biologia molecolare Struttura e dinamica di genomi e proteomi prima ed. (2018) ed. Zanichelli
Capranico G., Martegani E., Musci., Raugei G., Russo T., Zambrano N., Zappavigna. (2016) "Biologia Molecolare" ed. EdiSES
Watson JD, Baker TA, Bell SP, Gann A, Levine M. Losick R. "Biologia molecolare del gene" sesta ed (2014) ed. Zanichelli
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