Teacher
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TIEZZI Antonio
(syllabus)
Course Program of Biotechnology of Plant Molecules
What a plant is and what it is made up: a brief summary of the root, the stem, the leaf and the reproductive apparatus. Plant molecules have accompanied the path of humanity. What is a molecule. The cell is a set of molecules. The cell is a system in equilibrium, internal balance and in balance with the outside. The pathology as a result of altered balance. Primary metabolites, the molecules of all cells, and their biosynthesis: nucleic acids, proteins, lipids, polysaccharides, vitamins. Biological activities of primary metabolites. Methods for the extraction and purification of primary metabolites. Secondary metabolites. Classification of secondary metabolites and their biosynthesis: terpenes and terpenoids, phenolic compounds, alkaloids. Biological activities of secondary metabolites. Methods for the extraction and purification of secondary metabolites. The bioassay on bacterial, plant and animal cell models. What is the biological model; choice of biological model. Brief introduction to the identification and cultivation conditions of the biological model. Possible cytoplasmic targets of secondary metabolites.
Examples of human applications of molecules of plant origin in our daily lives. Examples of applications of plant molecules for environmental, animal and human health use.
In the laboratory. The search for new molecules. What is a research laboratory. Behavioral principles in a laboratory. The concept of the research group. The multidisciplinary approach to research. The research procedure in the field of plant molecules: the alternation and synergy of the chemical investigation and the biological investigation. The choice of plants to investigate; knowledge of the past and current knowledge, bibliographic research. Sample preparation. Chemical investigations: chromatography methods. Basics of mass spectrometry and nuclear magnetic resonance. Biological investigations: identification and culture of the biological model and sample administration; biological effect evaluation. What a scientific publication is and how it is structured. Writing a paper based on the results obtained from the laboratory experience
(reference books)
The Course has not referral texts The Teacher makes available all the slides showed during the Course, scientific articles, lecture notes.
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