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Code
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15414 |
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Language
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ITA |
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Type of certificate
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Profit certificate
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| Module: MODULE A
(objectives)
Learning results
The course will permit the comprehension of the morphological, physiological and metabolic characteristic of marine microorganisms (mainly prokaryotes); their role in marine environments and the interactions with other organisms. The course will permit to understand the principal interactions between marine microorganisms and the environment (with particular attention to some extreme marine environments) in relation with the bio-geochemical cycles. The course will supply a reference framework to comprehend modern aspect related to the marine microbial world and the potential applications of useful microorganisms.
Knowledge and understanding ability. To get the knowledge regarding the peculiarities of the marine microbial biology, their metabolism, their role in the marine environment, their interaction with other organisms, and their potential in biotechnology.
Ability to apply knowledge and understanding. To understand the issues related to the relationships between marine microorganism and environment (including other organisms). To acquire the knowledge regarding the experimental approach to study the marine microbial world.
Independent judgement. The ability to construe the results obtained by the various studies in marine microbiology. The ability to understand the role of the marine microorganisms in the environment and their influence on the biology of other organisms.
Learning ability. Ability to describe scientific topics related to marine microbiology both in written and oral forms by a specific scientific/technical language. Ability to use the methods acquired for microorganism sampling, recognition/identification and cultivation. This ability will be developed also trough the active involvement of the students in discussions during the lectures, by laboratory experiences and field activities.
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Language
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ITA |
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Type of certificate
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Profit certificate
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Credits
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6
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Scientific Disciplinary Sector Code
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BIO/19
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Contact Hours
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40
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Laboratory Hours
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8
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Type of Activity
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Core compulsory activities
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Teacher
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FENICE Massimiliano
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Dates of beginning and end of teaching activities
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From 04/03/2019 to 14/06/2019 |
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Attendance
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not mandatory
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| Module: MODULE B
(objectives)
The course will enable students to understand the peculiar morphological, physiological and metabolic characteristics of marine microorganisms (with particular reference to prokaryotes), their role in the marine environment and interactions with other organisms. Furthermore it will allow to know the basic techniques for their sampling and manipulation in the laboratory. The course will provide a framework for understanding current issues related to marine microorganisms and their potential applications (eg the importance of microorganisms for other marine organisms, their potential in biotechnology, the danger of some microorganisms and the necessary countermeasures to cancel potential damages of these). The course will provide the necessary insights to understand the ecological peculiarities of marine microorganisms, in particular those of some extreme marine environments.
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Language
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ITA |
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Type of certificate
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Profit certificate
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Credits
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6
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Scientific Disciplinary Sector Code
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CHIM/11
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Contact Hours
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40
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Laboratory Hours
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8
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Type of Activity
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Core compulsory activities
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Teacher
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BARGHINI Paolo
(syllabus)
Introduction to the course, knowledge verification test of basic microbiology. Classification of microorganisms. Classic characters and molecular characteristics as criteria for the classification of microorganisms. Identification of microorganisms. Physiological methods: Biolog. Molecular methods: DNA extraction; amplification; sequencing; databank comparison. Definition and construction of phylogenetic trees. Choice of method; choice of sequences and multialignment; outgroup choice. The bootstrap. Critical analysis of the results obtained. Recombinant DNA technology. Restriction enzymes and construction of a restriction map. Realization of a construct. Plasmids: cloning, expression, secretion, integration, shuttle vectors and for the study of the promoter vectors. Gene-libraries Construction. Theory of PCR. Evolution and applications. Real Time PCR; chemistry of detection systems; sybr green, hybridization probes or fret probes, hydrolysis probes or TaqMan probes. Applications: quantitative analysis; revelations of mutations; products characterization. Theoretical aspects of quantitative PCR. Calibration curve. Principles of quantification. Absolute and relative quantification. Analysis of the melting curve. Set-up of the PCR with the internal control. DNA sequencing with the Sanger method. New Generation Sequencing technologies (NGS). Prokaryotes genetic transformation. Treatment of competence. Chemistry Competence. Electroporation. Triparental conjugation. Mutations. Recombination: methods and mechanisms. Gene-conversion and unequal crossing over. Transposable elements (transposons): types structure and mechanisms of action. The Plasposon. Biofouling and quorum sensing. The sampling: theoretical and practical aspects for a correct sampling and statistically significant.
Sampling of marine microorganisms from anthropized and not anthropized environments.
Viable and total count of marine microorganisms.
Isolation, cultivation and classification (cultivation methods and molecular) of marine microorganisms.
Detection of microbial biodiversity of marine environments.
Biotechnology from the sea.
Screening for the production of metabolites / enzymes of industrial interest.
Microbiological analysis of seawater.
(reference books)
Colin Munn. Marine Microbiology: Ecology and Applications. Garland Science
Brock Biologia dei microorganismi: microbiologia generale, ambientale e industriale. 14° edizione (2016). Casa Editrice Ambrosiana.
Roberto Danovaro - Biologia Marina: biodiversità e funzionalità degli ecosistemi marini. CittàStudi Edizioni.
Barbieri, Bestetti, Galli, Zannoni- Microbiologia ambientale ed elementi di ecologia microbica - Casa Editrice Ambrosiana, edizione 2008.
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Dates of beginning and end of teaching activities
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From 04/03/2019 to 14/06/2019 |
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Delivery mode
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Traditional
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Attendance
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not mandatory
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Evaluation methods
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Oral exam
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